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Identification and bioinformatic analysis of Aux/IAA family based on transcriptome data of Bletilla striata
- Source :
- Bioengineered, article-version (VoR) Version of Record, Bioengineered, Vol 10, Iss 1, Pp 668-678 (2019)
- Publication Year :
- 2019
- Publisher :
- Taylor & Francis, 2019.
-
Abstract
- Auxin/Indole-3-Acetic Acid (Aux/IAA) genes are involved in auxin signaling pathway and play an important role in plant growth and development. However, many studies focus on Aux/IAA gene families and much less known in Bletilla striata. In this study, a total of 27 Aux/IAA genes (BsIAA1-27) were cloned from the transcriptome of Bletilla striata. Based on a phylogenetic analysis of the Aux/IAA protein sequences from B. striata, Arabidopsis thaliana and Dendrobium officinale, the Aux/IAA genes of B. striata (BsIAAs) were categorized into 2 subfamilies and 9 groups. While BsIAAs were more closer to those of D. officinale compared to A. thaliana. EST-SSR marker mining test showed that 4 markers could be stably amplified with obvious polymorphisms among 4 landraces. Our results suggested that BsIAAs were involved in the process of tuber development and provided insights into functional roles of Aux/IAA genes in B. striata and other plants.<br />Graphical Abstract In this paper, we identified the 27 members of the Aux/IAA gene family which can be divided into 2 groups and 9 subfamilies. The similar branches of Aux/IAA proteins had the same or similar motifs, such as genes between BsIAA10 and BsIAA12, BsIAA11 and BsIAA13, BsIAA18 and BsIAA20, indicating that Aux/IAA proteins were conserved. Among them, BsIAAs were more closer to those of D. officinale compared to A. thaliana.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Arabidopsis
01 natural sciences
Applied Microbiology and Biotechnology
Bletilla striata
Transcriptome
genome-wide
Plant Growth Regulators
Gene Expression Regulation, Plant
Arabidopsis thaliana
heterocyclic compounds
Phylogeny
Plant Proteins
Genetics
chemistry.chemical_classification
biology
Phylogenetic tree
food and beverages
Gene Expression Regulation, Developmental
Aux/IAA gene family
evolutionary analysis
General Medicine
Biological Evolution
Plant Tubers
Biotechnology
Research Article
Research Paper
Genetic Markers
lcsh:Biotechnology
Bioengineering
03 medical and health sciences
Auxin
lcsh:TP248.13-248.65
EST-SSR
Gene family
Amino Acid Sequence
Orchidaceae
Gene
bioinformatic
Indoleacetic Acids
Sequence Homology, Amino Acid
fungi
Computational Biology
biology.organism_classification
030104 developmental biology
chemistry
Family based
Dendrobium
Sequence Alignment
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 21655987 and 21655979
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....434d0d0fc1c07012b39383ab50eb0868