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Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues
- Source :
- Journal of the American Chemical Society, Journal of the American Chemical Society, American Chemical Society, 2018, 140 (16), ⟨10.1021/jacs.7b12817⟩
- Publication Year :
- 2018
-
Abstract
- Lipid droplets (LDs) are intracellular lipid-rich organelles that regulate the storage of neutral lipids and were recently found to be involved in many physiological processes, metabolic disorders, and diseases including obesity, diabetes, and cancers. Herein we present a family of new fluorogenic merocyanine fluorophores based on an indolenine moiety and a dioxaborine barbiturate derivative. These so-called StatoMerocyanines (SMCy) fluoresce from yellow to the near-infrared (NIR) in oil with an impressive fluorescence enhancement compared to aqueous media. Additionally, SMCy display remarkably high molar extinction coefficients (up to 390 000 M–1 cm–1) and high quantum yield values (up to 100%). All the members of this new family specifically stain the LDs in live cells with very low background noise. Unlike Nile Red, a well-known lipid droplet marker, SMCy dyes possess narrow absorption and emission bands in the visible, thus allowing multicolor imaging. SMCy proved to be compatible with fixation and le...
- Subjects :
- Models, Molecular
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
Indoles
multicolor imaging
02 engineering and technology
010402 general chemistry
01 natural sciences
Biochemistry
Catalysis
KB Cells
chemistry.chemical_compound
Mice
Colloid and Surface Chemistry
Lipid droplet
Microscopy
Organelle
Moiety
Animals
Humans
Merocyanine
Benzopyrans
Fluorescent Dyes
tissue imaging
communication
Chemistry
Optical Imaging
Nile red
dioxaborine
General Chemistry
Lipid Droplets
021001 nanoscience & nanotechnology
Fluorescence
Two-Photon Excitation imaging
0104 chemical sciences
Mice, Inbred C57BL
Adipose Tissue
Liver
Microscopy, Fluorescence
Biophysics
0210 nano-technology
Intracellular
Subjects
Details
- ISSN :
- 15205126 and 00027863
- Volume :
- 140
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- Journal of the American Chemical Society
- Accession number :
- edsair.doi.dedup.....43484f15c431ef3164ab75eb3b5b23bb