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Dual chemosensor for the rapid detection of mercury(ii) pollution and biothiols
- Source :
- The Analyst. 144(16)
- Publication Year :
- 2019
-
Abstract
- A new benzothiazole azo dye [(E)-1-((6-methoxybenzo[d]thiazole-2-yl)diazenyl)naphthalene-2,6-diol] (also known as “BAN”), has been synthesised and used as a chemosensor for the rapid and selective detection of mercury(II) ions in water. The pink coloured chemosensor turns blue when reacted with mercury(II) ions due to the formation of a 2 : 1 coordination complex. The complex formation causes a bathochromic shift of the chemosensor's UV absorption peak from 540 to 585 nm and turns on a highly selective fluorescence emission at 425 nm. The change in the optical property of BAN upon complexation with mercury(II) was confirmed by ab initio calculations. The new chemosensor was used to quantify mercury(II) ions in water by fluorescence spectroscopy down to 5 × 10−8 M (10 ppb). The limit of detection (LOD) of Hg2+ was 9.45 nM (1.8 ppb) which satisfies the maximum allowable Hg2+ concentration in drinking water that is set by the WHO. The BAN–Hg(II) complex was used for the determination of cysteine (Cys) in aqueous solution by UV-Vis spectroscopy down to 1 × 10−7 M. The thiol-containing amino acid preferentially coordinates the mercury ions of the BAN–Hg(II) complex. This causes dissociation of the blue-coloured complex and the liberation of the pink-coloured BAN dye. The colour change of the BAN–Hg(II) complex from blue to pink was selective to the Cys biothiol while other non-thiol containing amino acids did not cause a colour change. For the in-field application, filter paper strips were loaded with the BAN–Hg(II) complex and used as a disposable sensor for the detection of cysteine (Cys) by the naked eye. Therefore, the BAN chemosensor offers a sensitive, and rapid tool for the detection of mercury(II) in water. In addition, the BAN–Hg(II) complex can be used as a simple and selective chemosensor of the screening of purified biothiols, such cysetine, homocysteine and glutathione in biology research and pharmaceutical/food industries.
- Subjects :
- Cations, Divalent
chemistry.chemical_element
Color
02 engineering and technology
Ligands
01 natural sciences
Biochemistry
Fluorescence spectroscopy
Analytical Chemistry
Coordination complex
Coordination Complexes
Limit of Detection
Bathochromic shift
Electrochemistry
Environmental Chemistry
Computer Simulation
Benzothiazoles
Cysteine
Sulfhydryl Compounds
Spectroscopy
Density Functional Theory
Fluorescent Dyes
Detection limit
chemistry.chemical_classification
Aqueous solution
010401 analytical chemistry
Mercury
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Fluorescence
Glutathione
0104 chemical sciences
Mercury (element)
Spectrometry, Fluorescence
chemistry
Naked eye
0210 nano-technology
Water Pollutants, Chemical
Nuclear chemistry
Subjects
Details
- ISSN :
- 13645528
- Volume :
- 144
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....4326c8797462135a88c01ead660a895a