Analysis of localisation of megakaryocytes in the bone marrow of the rat - possible relationship with innervations

Piątek Małgorzata, Polaniak Renata, Tabarowski Zbigniew, Staśkiewicz Wiktoria, Kiciak Agata, Grajek Mateusz. Analysis of localisation of megakaryocytes in the bone marrow of the rat- possible relationship with innervations. Journal of Education, Health and Sport. 2022;12(7):479-492. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.07.049 https://apcz.umk.pl/JEHS/article/view/JEHS.2022.12.07.049 https://zenodo.org/record/6828868 The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 21, 2021. No. The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 21, 2021. No. 32343. Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences). Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 21 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159. Przypisane dyscypliny naukowe: Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu). © The Authors 2022; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 22.06.2022. Revised: 22.06.2022. Accepted: 13.07.2022. ANALYSIS OF LOCALISATION OF MEGAKARYOCYTES IN THE BONE MARROW OF THE RAT- POSSIBLE RELATIONSHIP WITH INNERVATIONS Małgorzata Piątek1, Renata Polaniak1*, Zbigniew Tabarowski2, Wiktoria Staśkiewicz3, Agata Kiciak3, Mateusz Grajek4 1Department of Human Nutrition, Department of Dietetics, Faculty of Health Sciences in Bytom, Medical University of Silesia in Katowice, Poland 2Department of Experimental Hematology, Department of Animal Physiology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Poland 3Department of Food Technology and Quality Evaluation, Department of Dietetics, Faculty of Health Sciences in Bytom, Medical University of Silesia in Katowice, Poland 4Department of Public Health, Department of Public Health Policy, Faculty of Health Sciences in Bytom, Medical University of Silesia in Katowice, Poland *Correspondence: Renata Polaniak, PhD; Jordana 19, 41808 Zabrze (Poland); rpolaniak@sum.edu.pl ABSTRACT Within the bone marrow, megakaryocytes thrive in an environment rich in numerous growth factors that influences their development, maturation, and may play a role in signaling the start of platelet production. Up to now, experiments concerning localisation of various populations of cells in rat bone marrow showed their certain relationship to the blood vessels. There are strikingly few reliable sources of information available in the literature that confirm the relationship between the localisation of these cells in connection to nerve fibers. Therefore the purpose of the present study was to examine if megakaryocytes are located in a defined relation to sensory and sympathetic nerve fibers. The study used immature 6 weeks rats of the Wistar breed of both sexes. The animals were kept under constant lighting conditions (12 hours light-dark cycle) and temperature, and had unrestricted access to water and food (standard laboratory rodent feed).Double immunostaining method was applied with usage of secondary antibodies conjugated with fluorochromes (Cy3 and DTAF) to identify cells. The antibody against CD42d was used to immunolocalise megakaryocytes, while in order to identify the distribution of nerves, antibodies anti-NPY (for detection sympathetic nerve fibers) and anti-PGP 9.5 (sensory nerve fibers) were applied. These findings showed the presence of megakaryocyte and megakaryoblastic cells which were distributed with a close relationship to the blood vessels but some of them were located in parenchyma. It was shown that NPY-positive and PGP 9.5-positive cells were present in the vicinity of blood cells. Furthermore, some megakaryocytes were located near PGP 9.5-labelled cells. The relationship between sympathetic nerve fibers containing neuropeptide Y and megakaryocytes was also detected. The presence of megakaryocytes with a close vicinity of nerve fibers suggests the influence of the released neurotransmitters on the location of the megakaryocytes in the bone marrow. KEYWORDS: megakaryocytes, platelets, bone marrow, innervation, neuropeptide Y, PGP 9.5