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Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips
- Source :
- Methods in Molecular Biology, Methods in Molecular Biology, 1661, pp.205-217, 2018, Selenoproteins, ⟨10.1007/978-1-4939-7258-6_15⟩, Selenoproteins, Selenoproteins, 1661, pp.205-217, 2018, 〈10.1007/978-1-4939-7258-6_15〉. 〈https://link.springer.com/protocol/10.1007/978-1-4939-7258-6_15〉, Methods in Molecular Biology ISBN: 9781493972579
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; In contrast to other trace elements that are cofactors of enzymes and removed from proteins under denaturing conditions, Se is covalently bound to proteins when incorporated into selenoproteins, since it is a component of selenocysteine aminoacid. It implies that selenoproteins can undergo several biochemical separation methods in stringent and chaotropic conditions and still maintain the presence of selenium in the primary sequence. This feature has been used to develop a method for the detection of trace levels of human selenoproteins in cell extracts without the use of radioactive isotopes. The selenoproteins are separated as a function of their isoelectric point (pI) using iso-electrofocusing (IEF) electrophoretic strips and detected by laser ablation-inductively coupled plasma mass spectrometry (LA-ICP MS). This method, therefore referred to as IEF-LA-ICP MS, allowed the detection of several selenoproteins in human cell lines, including Gpx1, Gpx4, TXNRD1, TXNRD2, and SELENOF.
- Subjects :
- 0301 basic medicine
ief
chemistry.chemical_element
selenof
Mass spectrometry
Tandem mass spectrometry
[ CHIM ] Chemical Sciences
03 medical and health sciences
chemistry.chemical_compound
icp ms
[CHIM]Chemical Sciences
selenium
glutathione peroxidase
Inductively coupled plasma mass spectrometry
cell culture
Chromatography
030102 biochemistry & molecular biology
Selenocysteine
integumentary system
thioredoxin reductase
Chaotropic agent
030104 developmental biology
Isoelectric point
chemistry
laser ablation
esi ms/ms
Immobilized pH gradient
Selenium
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-7257-9
- ISBNs :
- 9781493972579
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology, Methods in Molecular Biology, 1661, pp.205-217, 2018, Selenoproteins, ⟨10.1007/978-1-4939-7258-6_15⟩, Selenoproteins, Selenoproteins, 1661, pp.205-217, 2018, 〈10.1007/978-1-4939-7258-6_15〉. 〈https://link.springer.com/protocol/10.1007/978-1-4939-7258-6_15〉, Methods in Molecular Biology ISBN: 9781493972579
- Accession number :
- edsair.doi.dedup.....43057e46305a8443cbba8d7010f7264c
- Full Text :
- https://doi.org/10.1007/978-1-4939-7258-6_15⟩