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Connexin 43 expressed in endothelial cells modulates monocyte-endothelial adhesion by regulating cell adhesion proteins
Connexin 43 expressed in endothelial cells modulates monocyte-endothelial adhesion by regulating cell adhesion proteins
- Source :
- Molecular Medicine Reports. 12:7146-7152
- Publication Year :
- 2015
- Publisher :
- Spandidos Publications, 2015.
-
Abstract
- Adhesion between circulating monocytes and vascular endothelial cells is a key initiator of atherosclerosis. In our previous studies, it was demonstrated that the expression of connexin (Cx)43 in monocytes modulates cell adhesion, however, the effects of the expression of Cx43 in endothelial cells remains to be elucidated. Therefore, the present study investigated the role of the expression of Cx43 in endothelial cells in the process of cell adhesion. A total of four different methods with distinct mechanisms were used to change the function and expression of Cx43 channels in human umbilical vein endothelial cells: Cx43 channel inhibitor (oleamide), enhancer (retinoic acid), overexpression of Cx43 by transfection with pcDNA‑Cx43 and knock‑down of the expression of Cx43 by small interfering RNA against Cx43. The results indicated that the upregulation of the expression of Cx43 enhanced monocyte‑endothelial adhesion and this was markedly decreased by downregulation of Cx43. This mechanism was associated with Cx43‑induced expression of vascular cell adhesion molecule‑1 and intercellular cell adhesion molecule‑1. The effects of Cx43 in endothelial cells was independent of Cx37 or Cx40. These experiments suggested that local regulation of endothelial Cx43 expression within the vasculature regulates monocyte‑endothelial adhesion, a critical event in the development of atherosclerosis and other inflammatory pathologies, with baseline adhesion set by the expression of Cx43. This balance may be crucial in controlling leukocyte involvement in inflammatory cascades.
- Subjects :
- Cancer Research
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1
Biology
Biochemistry
Connexins
Monocytes
Cell Adhesion
Human Umbilical Vein Endothelial Cells
Genetics
medicine
Humans
Cell adhesion
Lymphocyte homing receptor
Molecular Biology
Cells, Cultured
Cell adhesion molecule
Monocyte
Soluble cell adhesion molecules
Gap Junctions
Adhesion
Cell biology
medicine.anatomical_structure
Oncology
Connexin 43
cardiovascular system
Molecular Medicine
Neural cell adhesion molecule
sense organs
biological phenomena, cell phenomena, and immunity
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....42fd0c7a04bc1b375cba38dea6d4ef1a
- Full Text :
- https://doi.org/10.3892/mmr.2015.4273