Additional file 2: of Examining the fundamental biology of a novel population of directly reprogrammed human neural precursor cells

Figure S2. The synthetic plasmid used in reprogramming does not integrate into drNPCs. RT-qPCR analysis demonstrates the lack of any plasmid sequences in reprogrammed drNPCs. 4 different primer pairs were designed to detect plasmid sequence (PCRs 1-4) and 1 ubiquitous promotor primer pair was used as positive control. Samples studied were control template (determined to be ~â 10 copy numbers), untransfected BMCs, and 3 distinct samples of drNPCs (drNPC1-3) made from different starting cells. UDâ =â undetected. (PDF 137 kb)