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Low performance of a SARS-CoV-2 point-of-care lateral flow immunoassay in symptomatic children during the pandemic
- Source :
- Jornal De Pediatria, Jornal de Pediatria v.98 n.2 2022, Jornal de Pediatria, Sociedade Brasileira de Pediatria (SBP), instacron:SBPE, Jornal de Pediatria, Volume: 98, Issue: 2, Pages: 136-141, Published: 20 APR 2022
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Objective To evaluate the accuracy of an antibody point-of-care lateral flow immunoassay (LFI - Wondfo Biotech Co., Guangzhou, China) in a pediatric population. Methods Children and adolescents (2 months to 18 years) with signs and symptoms suggestive of acute SARS-CoV-2 infection were prospectively investigated with nasopharyngeal RT-PCR and LFI at the emergency room. RT-PCR was performed at baseline, and LFI at the same time or scheduled for those with less than 7 days of the clinical picture. Overall accuracy, sensitivity and specificity were assessed, as well as according to the onset of symptoms (7-13 or ≥14 days) at the time of LFI test. Results In 175 children included, RT-PCR and LFI were positive in 51 (29.14%) and 36 (20.57%), respectively. The overall sensitivity, specificity, positive and negative predictive value was of 70.6% (95%CI 56.2-82.5), 96.8% (95%CI 91.9-99.1), 90.0% (95%CI 77.2-96.0), and 88.9% (95%CI 83.9-92.5), respectively. At 7-13 and ≥14 days after the onset of symptoms, sensitivity was 60.0% (95%CI 26.2-87.8) and 73.2% (95%CI 57.1-85.8) and specificity was 97.9% (95%CI 88.7-99.9) and 96.1% (95%CI 89.0-99.2), respectively. Conclusion Despite its high specificity, in the present study, the sensitivity of LFI in children was lower (around 70%) than most reports in adults. In acute care settings, although a positive result is informative, a negative LFI test cannot rule out COVID-19 in children.
- Subjects :
- Adult
medicine.medical_specialty
Adolescent
Coronavirus disease 2019 (COVID-19)
Point-of-Care Systems
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Point-of-care testing
Sensitivity and Specificity
03 medical and health sciences
COVID-19 Testing
0302 clinical medicine
Lateral flow immunoassay
030225 pediatrics
Acute care
Internal medicine
Pandemic
Humans
Medicine
030212 general & internal medicine
Child
Pandemics
Children
Point of care
Immunoassay
SARS-CoV-2
business.industry
COVID-19
Point-of-Care Testing
Pediatrics, Perinatology and Child Health
Original Article
business
Pediatric population
Subjects
Details
- ISSN :
- 00217557
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- Jornal de Pediatria
- Accession number :
- edsair.doi.dedup.....42da3bf10024fdd33b8692abee849781