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A paradoxical pro-apoptotic effect of thrombin on smooth muscle cells
- Source :
- Experimental cell research. 299(2)
- Publication Year :
- 2003
-
Abstract
- Whereas thrombin (below 10 nM) is a potent mitogen, recent studies report that exposure to higher doses of thrombin could lead to apoptosis of neurons and tumor cells. Our results show that prolonged exposure (≥24 h) to thrombin (50–100 nM) exerts a pro-apoptotic effect on cultured vascular smooth muscle cells (VSMCs). This phenomenon depends on thrombin serine-protease activity but is independent of PAR-1 and -4 activation and subsequent signaling. The parallel occurrence of cell retraction and cleavage of fibronectin suggests that thrombin-induced apoptosis is consecutive to pericellular proteolysis. These data point to a new potential action of thrombin in the cardiovascular system.
- Subjects :
- Male
Vascular smooth muscle
Cell
Apoptosis
Thrombomodulin
Cleavage (embryo)
Muscle, Smooth, Vascular
Thrombin
Transforming Growth Factor beta
medicine
In Situ Nick-End Labeling
Animals
Receptor, PAR-1
Cells, Cultured
biology
Intracellular Signaling Peptides and Proteins
Cell Biology
Peptide Fragments
Cell biology
Extracellular Matrix
Fibronectins
Rats
Fibronectin
medicine.anatomical_structure
Biochemistry
Rats, Inbred Lew
Mitogen-activated protein kinase
biology.protein
Matrix Metalloproteinase 2
Calcium
Apoptosis Regulatory Proteins
Carrier Proteins
circulatory and respiratory physiology
medicine.drug
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 299
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....42d0ede6aed62c8687fb748f3493b72f