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A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Stem Cell Research, Vol 39, Iss, Pp-(2019)
- Publication Year :
- 2019
-
Abstract
- Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.<br />We thank Mª José Román for technical assistance. This work was funded by grants from the Spanish Ministerio de Economía y Competitividad (MINECO: SAF2016-80419-R and CIBERNED: CB06/ 05/0065, PI2013/01 and PI2015-2/02-4) to C.V.
- Subjects :
- 0301 basic medicine
Apolipoprotein E
Genotyping Techniques
Biology
Sendai virus
Viral vector
Cell Line
03 medical and health sciences
Kruppel-Like Factor 4
0302 clinical medicine
Apolipoproteins E
SOX2
Polymorphism (computer science)
Genotype
Humans
Risk factor
Allele
lcsh:QH301-705.5
Embryoid Bodies
Genetics
Reverse Transcriptase Polymerase Chain Reaction
Cell Differentiation
Cell Biology
General Medicine
Immunohistochemistry
030104 developmental biology
lcsh:Biology (General)
Karyotyping
embryonic structures
Reprogramming
030217 neurology & neurosurgery
Developmental Biology
Microsatellite Repeats
Subjects
Details
- ISSN :
- 18767753
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Stem cell research
- Accession number :
- edsair.doi.dedup.....42cc4eb1c2ca91a74928bd2fbd4c5428