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A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles

Authors :
Carlos Vicario
César Rodríguez
Isabel Hernández
Eva Rodríguez-Traver
Itziar de Rojas
Elena P. Moreno-Jiménez
Eva Díaz-Guerra
Agustín Ruiz
Esther Arribas-González
María Orera
Ministerio de Economía y Competitividad (España)
Centro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España)
Source :
Digital.CSIC. Repositorio Institucional del CSIC, instname, Stem Cell Research, Vol 39, Iss, Pp-(2019)
Publication Year :
2019

Abstract

Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.<br />We thank Mª José Román for technical assistance. This work was funded by grants from the Spanish Ministerio de Economía y Competitividad (MINECO: SAF2016-80419-R and CIBERNED: CB06/ 05/0065, PI2013/01 and PI2015-2/02-4) to C.V.

Details

ISSN :
18767753
Volume :
39
Database :
OpenAIRE
Journal :
Stem cell research
Accession number :
edsair.doi.dedup.....42cc4eb1c2ca91a74928bd2fbd4c5428