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Micro-droplet-based calibration for quantitative elemental bioimaging by LA-ICPMS
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- In this work, a novel standardization strategy for quantitative elemental bioimaging is evaluated. More specifically, multi-element quantification by laser ablation-inductively coupled plasma-time-of-flight mass spectrometry (LA-ICP-TOFMS) is performed by multi-point calibration using gelatin-based micro-droplet standards and validated using in-house produced reference materials. Fully automated deposition of micro-droplets by micro-spotting ensured precise standard volumes of 400 ± 5 pL resulting in droplet sizes of around 200 μm in diameter. The small dimensions of the micro-droplet standards and the use of a low-dispersion laser ablation setup reduced the analysis time required for calibration by LA-ICPMS significantly. Therefore, as a key advance, high-throughput analysis (pixel acquisition rates of more than 200 Hz) enabled to establish imaging measurement sequences with quality control- and standardization samples comparable to solution-based quantification exercises by ICP-MS. Analytical figures of merit such as limit of detection, precision, and accuracy of the calibration approach were assessed for platinum and for elements with biological key functions from the lower mass range (phosphorus, copper, and zinc). As a proof-of-concept application, the tool-set was employed to investigate the accumulation of metal-based anticancer drugs in multicellular tumor spheroid models at clinically relevant concentrations. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03357-w.
- Subjects :
- Detection limit
Materials science
Laser ablation
Mass spectrometry
Spectrum Analysis
Laser
Bioimaging
Biochemistry
Analytical Chemistry
law.invention
law
Calibration
ICP-MS
Deposition (phase transition)
Figure of merit
Laser Therapy
Quantification strategy
Inductively coupled plasma mass spectrometry
Research Paper
Platinum
Biomedical engineering
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 414
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....42aafdd56749d6f26cf19b0f07c3710e