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Expression of the G protein-coupled estrogen receptor (GPER) in endometriosis: a tissue microarray study
- Source :
- Reproductive Biology and Endocrinology, Vol 10, Iss 1, p 30 (2012), Reproductive Biology and Endocrinology : RB&E
- Publication Year :
- 2012
- Publisher :
- BMC, 2012.
-
Abstract
- Background The G protein-coupled estrogen receptor (GPER) is thought to be involved in non-genomic estrogen responses as well as processes such as cell proliferation and migration. In this study, we analyzed GPER expression patterns from endometriosis samples and normal endometrial tissue samples and compared these expression profiles to those of the classical sex hormone receptors. Methods A tissue microarray, which included 74 samples from different types of endometriosis (27 ovarian, 19 peritoneal and 28 deep-infiltrating) and 30 samples from normal endometrial tissue, was used to compare the expression levels of the GPER, estrogen receptor (ER)-alpha, ER-beta and progesterone receptor (PR). The immunoreactive score (IRS) was calculated separately for epithelium and stroma as the product of the staining intensity and the percentage of positive cells. The expression levels of the hormonal receptors were dichotomized into low (IRS =6) expression groups. Results The mean epithelial IRS (+/−standard deviation, range) of cytoplasmic GPER expression was 1.2 (+/−1.7, 0–4) in normal endometrium and 5.1 (+/−3.5, 0–12) in endometriosis (p p = 0.71), of ER-alpha 10.6 (+/−2.4, 3–12) and 9.8 (+/−3.0, 2–12; p = 0.26), of ER-beta 2.4 (+/−2.2; 0–8) and 5.6 (+/−2.6; 0–10; p p p p = 0.001), of ER-beta 1.8 (+/−2.0; 0–8) and 5.4 (+/−2.5; 0–10; p p = 0.044), respectively. Cytoplasmic GPER expression was not detectable in the stroma of endometrium and endometriosis. The observed frequency of high epithelial cytoplasmic GPER expression levels was 50% (n = 30/60) in the endometriosis and none (0/30) in the normal endometrium samples (p p = 0.01), as compared to peritoneal (9/18, 50%) or deep-infiltrating endometriotic lesions (7/22, 31.8%). The frequency of high stromal nuclear GPER expression levels was 100% (n = 74/74) in endometriosis and 76.7% (n = 23/30) in normal endometrium (p Conclusions The present data indicate a unique GPER expression pattern in endometriosis, especially in endometriomas as compared to the normal endometrium. The overexpression of GPER in endometriotic lesions suggests a potential role for GPER in the hormonal regulation of endometriosis, which should be taken into consideration for future hormonal treatment strategies.
- Subjects :
- GPR30
Endometriosis
Estrogen receptor
Endometrium
Receptors, G-Protein-Coupled
Tissue microarray
1309 Developmental Biology
Endocrinology
lcsh:Reproduction
Obstetrics and Gynecology
Sex hormone receptor
Middle Aged
GPER
Immunohistochemistry
10174 Clinic for Gynecology
1310 Endocrinology
medicine.anatomical_structure
Receptors, Estrogen
Female
Receptors, Progesterone
Adult
medicine.medical_specialty
lcsh:QH471-489
medicine.drug_class
610 Medicine & health
lcsh:Gynecology and obstetrics
10049 Institute of Pathology and Molecular Pathology
Internal medicine
medicine
Estrogen Receptor beta
Humans
Estrogen receptor beta
lcsh:RG1-991
Retrospective Studies
business.industry
Research
Estrogen Receptor alpha
2729 Obstetrics and Gynecology
2743 Reproductive Medicine
medicine.disease
Reproductive Medicine
Tissue Array Analysis
Estrogen
business
Estrogen receptor alpha
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 14777827
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Reproductive Biology and Endocrinology
- Accession number :
- edsair.doi.dedup.....425d27050c8e32ecdb4d1ac6290d13fe