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Alterations of Fucosyltransferase Genes and Fucosylated Glycans in Gastric Epithelial Cells Infected with Helicobacter pylori
- Source :
- Pathogens, Volume 10, Issue 2, Pathogens, Vol 10, Iss 168, p 168 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Helicobacter pylori (H. pylori) adhesion to human gastric epithelial cells is closely linked with fucosylated glycans. Therefore, investigation of fucosylation in the interaction of gastric epithelial cells with H. pylori is critical. In this study we used lectin microarrays to detect the expression of fucosylated glycans in gastric epithelial cells (GES-1) infected with H. pylori strains isolated from patients with different diseases including chronic gastritis, duodenal ulcers, and gastric cancer (each containing two strains) at 4 h. In addition, we investigated the time-course expression of fucosyltransferase (FUT) 1–6 genes in GES-1 cells stimulated with H. pylori strains at 0.5–8 h. At 4 h post-infection, Lotus, AAA, BC2LCN, PA-IIL, CNL and ACG lectins had increased signals in H. pylori-infected GES-1 cells compared to uninfected cells. Higher expression of FUT1 and FUT2 was detected in all H. pylori-infected GES-1 cells within 2 h, regardless of the H. pylori strain. In particular, the expression of FUT2 was higher in H. pylori-infected GES-1 cells with a higher fold change in levels of BC2LCN lectin specific to α1-2 linked fucose (Fuc) at 4 h. The results suggest that the high levels of α1, 2-linked Fuc synthesized by FUT1/2, might play a role in the preliminary stage of H. pylori infection. This provides us with pivotal information to understand the adhesion of H. pylori to human gastric epithelial cells.
- Subjects :
- Microbiology (medical)
Glycan
Fucosyltransferase
lcsh:Medicine
Chronic gastritis
H. pylori
Fucose
chemistry.chemical_compound
medicine
Immunology and Allergy
Molecular Biology
Fucosylation
General Immunology and Microbiology
biology
fucosyltransferase expression
lcsh:R
Lectin
Helicobacter pylori
medicine.disease
biology.organism_classification
Molecular biology
Fold change
Infectious Diseases
chemistry
gastric epithelial cells
fucosylation
biology.protein
Subjects
Details
- ISSN :
- 20760817
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Pathogens
- Accession number :
- edsair.doi.dedup.....4254fa1788b313098f34a90ab8614053