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Gynura divaricata Water Extract Presented the Possibility to Enhance Neuronal Regeneration
- Source :
- Evidence-based Complementary and Alternative Medicine : eCAM, Evidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
- Publication Year :
- 2021
- Publisher :
- Hindawi, 2021.
-
Abstract
- Gynura divaricata (GD) is an Asian herb widely used as an alternative medicine and functional food for type 2 diabetes. Diabetic neuropathy is considered as an important complication of diabetic patients. This study focused on neuroregenerative effects of GD for use in the prevention of diabetic neuropathy. GD leaves were cut and boiled in water to mimic real-life cooking. The boiled content was filtered through white gauze and lyophilized to preserve as dried powder. Antioxidant assay was performed using DPPH assays. UHPLC-QTOF-MS/MS was employed to test for important compounds in the extract of these herbs. MTT assay was used to test for cell viability. The extracts at concentration of 250 μg/mL were tested with human gingival cell to observe the change of gene expression. The DPPH assay showed that GD water extract at the concentration of 5000 μg/mL could inhibit DPPH radical for 39.2%. The results showed that 5000 µg of GD water extract contained total phenolic content equivalent to 310.9 µg standard gallic acid. UHPLC-QTOF-MS/MS result found phenolic acids and flavonoids as the main components. Human gingival cells treated with 250 μg/mL of GD water extract for 10 days showed upregulation of some neuronal differentiation markers. Staining with Cdr3 dye confirmed the presentation of neuronal progenitors. The extract at the concentration of 250 μg/mL was also tested with apical papilla cells to screen for change of gene expression by RNA sequencing. The result also showed significant upregulation of alpha-internexin (INA). These results indicated that GD water extract might have an inductive effect for neural regeneration and could be used as functional food and supplementation for the prevention or treatment of diabetic neuropathy. This work provided the basic knowledge for further investigations into the benefits of GD for diabetic neuropathy.
- Subjects :
- Antioxidant
Diabetic neuropathy
Article Subject
DPPH
medicine.medical_treatment
Pharmacology
03 medical and health sciences
chemistry.chemical_compound
Other systems of medicine
0302 clinical medicine
Functional food
medicine
MTT assay
Gallic acid
Gynura
Viability assay
030304 developmental biology
0303 health sciences
biology
biology.organism_classification
medicine.disease
Complementary and alternative medicine
chemistry
030220 oncology & carcinogenesis
RZ201-999
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- OpenAIRE
- Journal :
- Evidence-Based Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....424ee73978a136a3a487c2e6ebc7bbbc
- Full Text :
- https://doi.org/10.1155/2021/8818618