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Intestinal microbiota diversity of the flat fish Solea senegalensis (Kaup, 1858) following probiotic administration
- Source :
- e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía, instname
- Publication Year :
- 2009
-
Abstract
- Pleuronectiforms are an important group of fish, and one of their species, Solea senegalensis (Kaup 1858), has been extensively studied at different levels, although information about its intestinal microbiota and the effects of different factors on it is very scarce. Modern aquaculture industry demands strategies which help to maintain a microbiologically healthy environment and an environmentally friendly aquaculture. In this context, probiotics seem to offer an attractive alternative. The intake of probiotics could modify the composition of the intestinal microbiota, which is a key component in excluding potential invaders and maintaining health. The aim of this study was to evaluate by 16S rRNA gene analysis using polymerase chain reaction-denaturing gradient gel electrophoresis the effect of administering fresh or lyophilized cells of Pdp11 on the intestinal microbiota of farmed Senegalese sole, using sodium alginate to facilitate the incorporation of bacterial cells to the feed. The results obtained showed that the composition of fish intestinal microbiota was affected when fish received a diet supplemented with sodium alginate and fresh or lyophilized probiotic cells. In all cases, the dominant bacterial groups belonged to γ-Proteobacteria and mainly the Vibrio species. The use of sodium alginate reduced the incidence of populations with<br />Sí
- Subjects :
- DNA, Bacterial
Acuicultura
Centro Oceanográfico de Santander
Fish farming
Soil Science
Context (language use)
Biology
Polymerase Chain Reaction
Microbiology
law.invention
Probiotic
Aquaculture
Microbial ecology
law
RNA, Ribosomal, 16S
Animals
Ecology, Evolution, Behavior and Systematics
Phylogeny
Vibrio
Ecology
business.industry
Denaturing Gradient Gel Electrophoresis
Probiotics
Aquatic animal
Sequence Analysis, DNA
biology.organism_classification
Intestines
Flatfishes
Metagenome
business
Temperature gradient gel electrophoresis
Subjects
Details
- ISSN :
- 1432184X
- Volume :
- 60
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Microbial ecology
- Accession number :
- edsair.doi.dedup.....4246911f56acb219b45f9d64b1325322