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Characterization of 24 microsatellite loci in delta smelt, Hypomesus transpacificus, and their cross-species amplification in two other smelt species of the Osmeridae family
- Source :
- Molecular ecology resources. 9(1)
- Publication Year :
- 2011
-
Abstract
- We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus ) endemic to the San Francisco Bay Estuary, California, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy–Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy–Weinberg equilibrium. Twenty-two primer pairs crossamplified in wakasagi smelt ( Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt (Spirinchus thaleichthys).
Details
- ISSN :
- 1755098X
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular ecology resources
- Accession number :
- edsair.doi.dedup.....4237900d5898d300642bba1ab938fa17