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Investigation of Pokemon-regulated proteins in hepatocellular carcinoma using mass spectrometry-based multiplex quantitative proteomics
- Source :
- European journal of mass spectrometry (Chichester, England). 19(2)
- Publication Year :
- 2013
-
Abstract
- Pokemon is a transcription regulator involved in embryonic development, cellular differentiation and oncogenesis. It is aberrantly overexpressed in multiple human cancers including Hepatocellular carcinoma (HCC) and is considered as a promising biomarker for HCC. In this work, the isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics strategy was used to investigate the proteomic profile associated with Pokemon in human HCC cell line QGY7703 and human hepatocyte line HL7702. Samples were labeled with four-plex iTRAQ reagents followed by two-dimensional liquid chromatography coupled with tandem mass spectrometry analysis. A total of 24 differentially expressed proteins were selected as significant. Nine proteins were potentially up-regulated by Pokemon while 15 proteins were potentially down-regulated and many proteins were previously identified as potential biomarkers for HCC. Gene ontology (GO) term enrichment revealed that the listed proteins were mainly involved in DNA metabolism and biosynthesis process. The changes of glucose-6-phosphate 1-dehydrogenase (G6PD, up-regulated) and ribonucleoside-diphosphate reductase large sub-unit (RIM1, down-regulated) were validated by Western blotting analysis and denoted as Pokemon's function of oncogenesis. We also found that Pokemon potentially repressed the expression of highly clustered proteins (MCM3, MCM5, MCM6, MCM7) which played key roles in promoting DNA replication. Altogether, our results may help better understand the role of Pokemon in HCC and promote the clinical applications.
- Subjects :
- Proteomics
Carcinoma, Hepatocellular
Ribonucleoside Diphosphate Reductase
Transcription, Genetic
Cellular differentiation
Quantitative proteomics
Blotting, Western
Computational biology
Glucosephosphate Dehydrogenase
Tandem mass spectrometry
medicine.disease_cause
Transfection
Mass Spectrometry
GTP-Binding Proteins
Cell Line, Tumor
medicine
Humans
RNA, Small Interfering
Spectroscopy
Proteomic Profile
biology
MCM6
Liver Neoplasms
DNA replication
General Medicine
Molecular biology
Atomic and Molecular Physics, and Optics
Blot
DNA-Binding Proteins
Data Interpretation, Statistical
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
biology.protein
Indicators and Reagents
Carcinogenesis
Plasmids
Transcription Factors
Subjects
Details
- ISSN :
- 14690667
- Volume :
- 19
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- European journal of mass spectrometry (Chichester, England)
- Accession number :
- edsair.doi.dedup.....42098e4920ff1a4cd6ac8c835593ed0a