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Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster
- Source :
- Ecotoxicology. 19:1440-1451
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Although mounting evidence suggests exposure to estrogenic contaminants increases vitellogenin production in molluscs, demonstration of dose-response relationships and knowledge of the temporal nature of the vitellogenin response with continual exposure is currently lacking for biomarker utility. To address this knowledge gap, adult Sydney rock oysters, Saccostrea glomerata, were exposed to a range of environmentally relevant concentrations of 17α-ethynylestradiol (EE2) (0, 6.25, 12.5, 25 or 50 ng/l) in seawater under laboratory conditions. Vitellogenin induction and gonadal development was assessed following 4, 21 and 49 days exposure to EE2. Vitellogenin was found to increase in a dose dependent manner with EE2 exposure for females (4 and 49 days) and males (4 and 21 days). Histological examination of gonads revealed a number of individuals exhibited intersex (ovotestis) in 50 ng/l EE2 (after 21 days) and in 6.25 and 12.5 ng/l EE2 (after 49 days). Furthermore, a significant shift towards females was observed following 49 days exposure at 50 ng/l EE2 suggesting estrogenic exposure is capable of facilitating a progression for protandric males from male-intersex-female gametal status. Increases in female vitellogenin (4 days) were predictive of later increases in female developmental stages at 21 days and increases in oocyte area following 49 days. Male vitellogenin (4 days) was predictive of decreased male percentages and lower male developmental stages at 49 days. Vitellogenin in S. glomerata is a predictive biomarker of estrogenic exposure and effect if sampled soon after exposure and at the commencement of a gonadal development cycle.
- Subjects :
- Male
endocrine system
Sydney rock oyster
Time Factors
Health, Toxicology and Mutagenesis
Disorders of Sex Development
Management, Monitoring, Policy and Law
Ethinyl Estradiol
Toxicology
Andrology
Vitellogenins
Vitellogenin
Sex Factors
medicine
Animals
17α-ethynylestradiol
Gonads
Histological examination
Dose-Response Relationship, Drug
Ovotestis
biology
Estrogens
General Medicine
biology.organism_classification
Oocyte
Ostreidae
medicine.anatomical_structure
Oocytes
biology.protein
Biomarker (medicine)
Female
Development of the gonads
Biomarkers
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 15733017 and 09639292
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Ecotoxicology
- Accession number :
- edsair.doi.dedup.....4201bda0289fefe300d09c8a1c53eeb3
- Full Text :
- https://doi.org/10.1007/s10646-010-0529-5