[ELISA kit for detection of biliary vesicular protein and its preliminary clinical application]

ObjectiveTo establish a rapid and precise detectiv e method of 33.5 kd vesicular protein and to screen an effective treatment of chol elithiasis. MethodsSpecific antibody of the biliary vesicular prot ein was obtained by immunizing rabbits and enzyme-linked immunosorbent assay (E LISA) kit was developed. The concentrations of 33.5 kd vesicular protein in seru m and bile of gallstone patients and control were examined respectively. The eff ects of Cholagogue Dry Syrup and Eulektrol Capsule on decreasing 33.5 kd vesicul ar protein were also studied by ELISA kit. ResultsOne-step ELISA equation was Y= 0.035 X (r=0.99).The vesicular protein concentrations in seru m and bile of cholesterol gallstone group (179.8±97.9 ) mg/L and (213.4±70. 1)mg/L respectively were significantly (P 0.05) higher than in the pigme nt stone group and control. Data showed that, with 2-week administration, Chola gogue Dry Syrup significantly decreased both biliary and serum 33.5 kd vesicular protein of cholesterol gallstone patients, while Eulekrol Capsule and control g roups didn’t have the same results. Conclusion The concentrations o f 33.5 kd protein are different in cholesterol gallstone patients and healthy gr oups which might be related to cholesterol nucleation process. Cholagogue Dry Sy rup is of cholagogic and litholytic effect by decreasing biliary lithogenesis.