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Comparison of neuronal death and expression of TNF‑α and MCT4 in the gerbil hippocampal CA1 region induced by ischemia/reperfusion under hyperthermia to those under normothermia
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2020
- Publisher :
- Spandidos Publications, 2020.
-
Abstract
- Monocarboxylate transporter 4 (MCT4) is a high‑capacity lactate transporter in cells and the alteration in MCT4 expression harms cellular survival. The present study investigated whether hypothermia affects tumor necrosis factor‑α (TNF‑α) and MCT4 immunoreactivity in the subfield cornu ammonis 1 (CA1) following cerebral ischemia/reperfusion (IR) in gerbils. Hypothermia was induced for 30 min before and during ischemia. It was found that IR‑induced death of pyramidal neurons was markedly augmented and occurred faster under hyperthermia than under normothermia. TNF‑α immunoreactivity in the pyramidal cells started to increase at 3 h after IR and peaked at 1 day after IR under normothermia. However, in hyperthermic control and sham operated gerbils, TNF‑α immunoreactivity was significantly increased compared with the normothermic gerbils, and IR under hyperthermia caused a more rapid and significant increase in TNF‑α immunoreactivity in pyramidal neurons than under normothermia. In addition, in the normothermic gerbils, MCT4 immunoreactivity began to decrease in pyramidal neurons from 3 h after IR and markedly increased at 1 and 2 days after IR. On the other hand, MCT4 immunoreactivity in pyramidal neurons of the hyperthermic gerbils was significantly increased from 3 h after IR, maintained until 1 day after IR and markedly decreased at 2 days after IR. These results indicate that acceleration of IR‑induced neuronal death under hyperthermia might be closely associated with early alteration of TNF‑α and MCT4 protein expression in the gerbil hippocampus after IR.
- Subjects :
- Male
Monocarboxylic Acid Transporters
0301 basic medicine
Hyperthermia
Cancer Research
medicine.medical_specialty
Ischemia
Hippocampus
hippocampal CA1 region
Hippocampal formation
Gerbil
Biochemistry
Brain Ischemia
03 medical and health sciences
0302 clinical medicine
Internal medicine
Genetics
medicine
Animals
CA1 Region, Hippocampal
Molecular Biology
Neurons
Monocarboxylate transporter
Cell Death
biology
Tumor Necrosis Factor-alpha
Chemistry
Pyramidal Cells
Articles
Hypothermia
monocarboxylate transporter 4
Fluoresceins
medicine.disease
neuronal death
hyperthermic condition
Disease Models, Animal
030104 developmental biology
Endocrinology
nervous system
Oncology
Reperfusion Injury
030220 oncology & carcinogenesis
biology.protein
Monocarboxylate transporter 4
Molecular Medicine
medicine.symptom
Gerbillinae
transient forebrain ischemia
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....41f02435b9e4f674584404272c44c77f