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DNA methylation of indoleamine 2,3-dioxygenase 1 (IDO1) in head and neck squamous cell carcinomas correlates with IDO1 expression, HPV status, patients’ survival, immune cell infiltrates, mutational load, and interferon γ signature
- Source :
- EBioMedicine, Vol 48, Iss, Pp 341-352 (2019), EBioMedicine
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Background: The immune checkpoint, indoleamine 2,3-dioxygenase 1, is under investigation as target of novel immunotherapies for cancers, including head and neck squamous cell carcinomas (HNSCC). The aim of our study was to analyze DNA methylation of the encoding gene (IDO1) in HNSCC. Methods: Methylation of three CpG sites within the promoter, promoter flank, and gene body was investigated and correlated with mRNA expression, immune cell infiltration, mutational burden, human papillomavirus (HPV)-status, and overall survival in a cohort of N = 528 HNSCC patients obtained from The Cancer Genome Atlas. In addition, IDO1 immunohistochemistry and DNA methylation analysis was performed in an independent cohort of N = 138 HNSCC samples. Findings: Significant inverse correlations of IDO1 methylation and IDO1 mRNA expression were found in the promoter and promoter flank region (Spearman's ρ = −0.163 and ρ = −0.377, respectively) while a positive correlation was present in the gene body (ρ = 0.502; all P
- Subjects :
- 0301 basic medicine
Male
HPV
Research paper
medicine.medical_treatment
lcsh:Medicine
Kaplan-Meier Estimate
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
medicine
Biomarkers, Tumor
Cluster Analysis
Humans
Indoleamine-Pyrrole 2,3,-Dioxygenase
Indoleamine 2,3-dioxygenase 1
RNA, Messenger
Indoleamine 2,3-dioxygenase
Gene
Papillomaviridae
lcsh:R5-920
DNA methylation
Squamous Cell Carcinoma of Head and Neck
Papillomavirus Infections
lcsh:R
Head and neck squamous cell carcinoma
General Medicine
Methylation
Immunotherapy
medicine.disease
Prognosis
Head and neck squamous-cell carcinoma
Immunohistochemistry
Immune checkpoint
Gene Expression Regulation, Neoplastic
030104 developmental biology
CpG site
030220 oncology & carcinogenesis
Cancer research
Cytokines
CpG Islands
Female
Prediction
lcsh:Medicine (General)
IDO1, Biomarker
Subjects
Details
- Language :
- English
- ISSN :
- 23523964
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- EBioMedicine
- Accession number :
- edsair.doi.dedup.....41e574bdbe6769a24b375d551ebb6dba