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Bis(hydroxyphenyl)methane-bisphenol F-metabolism by the HepG2 human hepatoma cell line and cryopreserved human hepatocytes
- Source :
- Drug and Chemical Toxicology, Drug and Chemical Toxicology, Taylor & Francis, 2011, 34 (4), pp.445-453. ⟨10.3109/01480545.2011.585651⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- author cannot archive publisher's version/PDF; International audience; Bisphenol F (BPF) is present in the environment and as a contaminant of food. Humans may, therefore, be exposed to BPF, and an assessment of this risk is required. BPF has been shown to have genotoxic and endocrine-disruptor properties in a human hepatoma cell line (HepG2), which is a model system for studies of xenobiotic toxicity. In this study, we investigated the ability of HepG2 cells to biotransform BPF, because metabolism may affect the observed effects of BPF, and we compared this metabolic capacity with that of human hepatocytes. Cells were incubated for 24 hours with [(3)H]-BPF. The culture medium was then concentrated and its metabolites were isolated by high-performance liquid chromatography and identified by mass spectrometry. BPF was largely metabolized into the corresponding sulfate by the HepG2 cell line. BPF was metabolized into both sulfate and glucuronide by human hepatocytes, but with differences between individuals. The metabolism of BPF in both HepG2 cells and human hepatocytes suggests the existence of a detoxification pathway. Thus, these two cell models differ in metabolic capacity. It is, therefore, very important, when assessing the toxic effects of substances in vitro, to determine, in parallel, the biotransformation capacities of the model used to extrapolate in vivo.
- Subjects :
- Bisphenol F
Health, Toxicology and Mutagenesis
estrogenicity
Cell Culture Techniques
010501 environmental sciences
Toxicology
01 natural sciences
Mass Spectrometry
Cryopreservation
chemistry.chemical_compound
enzyme level
[SDV.IDA]Life Sciences [q-bio]/Food engineering
performance liquid chromatography
rat
Luciferases
induction
Chromatography, High Pressure Liquid
endocrine disruptor
0303 health sciences
food and environmental contaminant
Molecular Structure
Hep G2 Cells
General Medicine
Biochemistry
Hepg2 cells
in vitro model
disposition
Toxicity
Environmental Pollutants
liver enzyme
biotransformation
Glucuronide
Plasmids
Biology
Transfection
liver
03 medical and health sciences
Humans
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Benzhydryl Compounds
030304 developmental biology
0105 earth and related environmental sciences
Pharmacology
Chemical Health and Safety
activity
bisphenol a
Estrogen Receptor alpha
Public Health, Environmental and Occupational Health
Metabolism
beta-Galactosidase
Hepatoma cell line
chemistry
Hepatocytes
Xenobiotic
Subjects
Details
- Language :
- English
- ISSN :
- 01480545 and 15256014
- Database :
- OpenAIRE
- Journal :
- Drug and Chemical Toxicology, Drug and Chemical Toxicology, Taylor & Francis, 2011, 34 (4), pp.445-453. ⟨10.3109/01480545.2011.585651⟩
- Accession number :
- edsair.doi.dedup.....41c1fdc7dd4513791803caaeec8a59d6