Back to Search
Start Over
circRNA-Mediated Inhibin–Activin Balance Regulation in Ovarian Granulosa Cell Apoptosis and Follicular Atresia
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 17, International Journal of Molecular Sciences, Vol 22, Iss 9113, p 9113 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Ovarian granulosa cells (GC) play an essential role in the development and atresia of follicles. Emerging studies suggest that non-coding RNAs are involved in the regulation of GC apoptosis. Here, we aimed to analyze the function of ssc-circINHA-001, coded by the first exon of the inhibin subunit α gene (INHA), in resisting GC apoptosis and follicular atresia by enhancing the expression of the inhibin subunit β A (INHBA) through a cluster of miRNAs. A higher expression of ssc-circINHA-001 in healthy follicles compared to early atretic follicles was detected by qRT-PCR. Its circular structure was confirmed by RNase R treatment and reversed PCR. The function of ssc-circINHA-001 in GC resistance to apoptosis was detected by in vitro transfection of its si-RNA. Furthermore, the dual-luciferase reporter assay suggested that ssc-circINHA-001 adsorbed three miRNAs, termed miR-214-5p, miR-7144-3p, and miR-9830-5p, which share the common target INHBA. A low expression of ssc-circINHA-001 increased the levels of the free miRNAs, inhibited INHBA expression, and thus raised GCs apoptosis through a shift from the secretion of activin to that of inhibin. Our study demonstrated the existence of a circRNA–microRNAs–INHBA regulatory axis in follicular GC apoptosis and provides insight into the relationship between circRNA function and its coding gene in inhibin/activin balance and ovarian physiological functions.
- Subjects :
- endocrine system
Ovarian Granulosa Cell
QH301-705.5
Follicular Atresia
Sus scrofa
Apoptosis
Biology
Catalysis
Article
INHBA
Inorganic Chemistry
Exon
INHA
Follicular phase
microRNA
Animals
inhibin
Inhibins
circRNA
Biology (General)
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Spectroscopy
miRNA
Reporter gene
Granulosa Cells
Follicular atresia
Organic Chemistry
Ovary
granulosa cell apoptosis
activin
General Medicine
RNA, Circular
Computer Science Applications
Cell biology
Activins
Chemistry
MicroRNAs
Gene Expression Regulation
Female
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....41b219327b069d2b830ed0c48c8e250b
- Full Text :
- https://doi.org/10.3390/ijms22179113