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miR-20a-5p promotes pulmonary artery smooth muscle cell proliferation and migration by targeting ABCA1
- Source :
- Journal of biochemical and molecular toxicologyREFERENCES. 34(12)
- Publication Year :
- 2020
-
Abstract
- Background The function of miR-20a-5p in pulmonary artery smooth muscle cells (PASMCs) and the underlying mechanism remains largely unknown. Methods C57BL/6J mice and PASMCs were used for constructing pulmonary artery hypertension (PAH) animal and cell models, respectively. Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect miR-20a-5p and ATP-binding cassette subfamily A member 1 (ABCA1) messenger RNA expression. CCK-8, Transwell, and TUNEL experiments were used to determine PASMCs proliferation, migration, and apoptosis. The relationship between miR-20a-5p and ABCA1 was detected by luciferase reporter experiment, Western blot analysis, and qRT-PCR. Results miR-20a-5p was remarkably elevated in PASMCs of PAH mice and human PASMCs treated by hypoxia, while ABCA1 was remarkably decreased. After transfection of miR-20a-5p mimics, PASMCs proliferation and migration were promoted and PASMCs apoptosis was suppressed. ABCA1 was confirmed to be a target of miR-20a-5p and restoration of ABCA1 reversed the function of miR-20a-5p. Conclusion miR-20a-5p enhances the proliferation and migration of PASMCs to promote the development of PAH via targeting ABCA1.
- Subjects :
- 0301 basic medicine
Health, Toxicology and Mutagenesis
Cell
Apoptosis
Pulmonary Artery
Toxicology
Biochemistry
Muscle, Smooth, Vascular
03 medical and health sciences
Mice
0302 clinical medicine
Western blot
Cell Movement
polycyclic compounds
medicine
Animals
Humans
Molecular Biology
Cells, Cultured
Cell Proliferation
TUNEL assay
030102 biochemistry & molecular biology
medicine.diagnostic_test
Chemistry
Cell growth
General Medicine
Transfection
Hypoxia (medical)
Cell biology
Reverse transcription polymerase chain reaction
Mice, Inbred C57BL
MicroRNAs
medicine.anatomical_structure
030220 oncology & carcinogenesis
Molecular Medicine
medicine.symptom
ATP Binding Cassette Transporter 1
Subjects
Details
- ISSN :
- 10990461
- Volume :
- 34
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of biochemical and molecular toxicologyREFERENCES
- Accession number :
- edsair.doi.dedup.....419d03a18ead2a3c7603b522be78040d