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The Effect of Metformin in Diabetic and Non-Diabetic Rats with Experimentally-Induced Chronic Kidney Disease
- Source :
- Biomolecules, Volume 11, Issue 6, Biomolecules, Vol 11, Iss 814, p 814 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- This work aimed to investigate whether treatment with the antidiabetic drug metformin would affect adenine-induced chronic kidney disease (CKD) in non-diabetic rats and rats with streptozotocin (STZ)-induced diabetes. Rats were randomly divided into eight groups, and given either normal feed, or feed mixed with adenine (0.25% w/w, for five weeks) to induce CKD. Some of these groups were also simultaneously treated orally with metformin (200 mg/kg/day). Rats given adenine showed the typical signs of CKD that included detrimental changes in several physiological and traditional and novel biochemical biomarkers in plasma urine and kidney homogenates such as albumin/creatinine ratio, N-acetyl-beta-D-glucosaminidase, neutrophil gelatinase-associated lipocalin, 8-isoprostane, adiponectin, cystatin C, as well as plasma urea, creatinine, uric acid, indoxyl sulfate, calcium, and phosphorus. Several indices of inflammation and oxidative stress, and renal nuclear factor-κB and nuclear factor erythroid 2-related factor 2 levels were also measured. Histopathologically, adenine caused renal tubular necrosis and fibrosis. The activation of the intracellular mitogen-activated protein kinase signaling pathway was inhibited in the groups that received metformin and STZ together, with or without adenine induced-CKD. Induction of diabetes worsened most of the actions induced by adenine. Metformin significantly ameliorated the renal actions induced by adenine and STZ when these were given singly, and more so when given together. The results suggest that metformin can be a useful drug in attenuating the progression of CKD in both diabetic and non-diabetic rats.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
endocrine system diseases
MAP Kinase Signaling System
Kidney
Microbiology
Biochemistry
Article
Diabetes Mellitus, Experimental
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Diabetes mellitus
Medicine
Animals
Rats, Wistar
Renal Insufficiency, Chronic
Molecular Biology
adenine
Creatinine
biology
diabetes
business.industry
nutritional and metabolic diseases
medicine.disease
Streptozotocin
QR1-502
Metformin
rats
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Cystatin C
030220 oncology & carcinogenesis
biology.protein
Uric acid
business
metformin
chronic kidney disease
medicine.drug
Kidney disease
Subjects
Details
- Language :
- English
- ISSN :
- 2218273X
- Database :
- OpenAIRE
- Journal :
- Biomolecules
- Accession number :
- edsair.doi.dedup.....418b4e0fb4ef94c40b46dc777bf9a49a
- Full Text :
- https://doi.org/10.3390/biom11060814