Immunomodulatory, antioxidant and intestinal morphology-regulating activities of alfalfa polysaccharides in mice

The effects of alfalfa polysaccharides (APS) on immunomodulatory and antioxidant functions, as well as intestinal morphology were investigated in vivo in this study. Sixty-four mice were randomly divided into four groups and administered 0, 200, 400 or 800 mg/kg/d body weight APS via gavage for 28 days. The blood parameters and metabolites, viscera indices, antioxidant enzyme activities and intestinal morphology were measured. The results showed that the oral administration of APS improved the immune functions of mice, significantly enhanced the white blood cells and lymphocyte counts, and led to improvements in spleen and thymus indices. APS exhibited significant antioxidant activity by enhancing total antioxidant capacity, superoxide dismutase and glutathione peroxidase activities in heart, kidney and liver, and decreasing the malondialdehyde levels of heart and liver. Moreover, administration of APS potently enhanced the small intestinal villous height and the villus-to-crypt ratio, and decreased the crypt depth of duodenum in mice. Therefore, we can conclude that APS possesses pronounced immunomodulatory activities, and plays an important role in the prevention of oxidative stresses and in the improvement of intestinal morphology in the immunological system in vivo. APS thus shows potential for the development as an effective natural immunomodulatory and antioxidant agent.