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Screen-printed electrodes modified with carbon black and polyelectrolyte films for determination of cancer marker carbohydrate antigen 19-9

Authors :
Gustavo Freitas do Nascimento
Rui Manuel Reis
André Lopes Carvalho
Deivy Wilson
Roberto H. M. Furuta
Gisela Ibáñez-Redín
Débora Gonçalves
Osvaldo N. Oliveira
Elsa M. Materon
Matias Eliseo Melendez
Source :
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
Publication Year :
2020

Abstract

Electrochemical immunosensors have been developed to determine the carbohydrate antigen 19-9 (CA19-9). They are based on screen-printed carbon electrodes (SPCEs) coated with layer-by-layer (LbL) films of carbon black (CB) and polyelectrolytes. Owing to a suitable choice of LbL film architecture, the procedures for immobilization of anti-CA19-9 antibodies on the electrode surfaces were straightforward. Mechanically flexible immunosensors were capable of detecting CA19-9 within a dynamic range of 0.01 to 40 U mL−1 and a limit of detection of 0.07 U mL−1 using differential pulse voltammetry. In addition to detecting CA19-9 at clinically relevant concentrations for pancreatic cancer in standard solutions, the immunosensors provide the determination of CA19-9 on cell lysate and human serum samples. Using LbL films led to immunosensors with superior performance compared to similar systems obtained by drop casting. The fabrication of this relatively simple, inexpensive platform is a demonstration that SPCEs modified with cost-effective materials are able to detect cancer biomarkers and may be adapted to other disposable immunosensors.

Details

ISSN :
14365073
Volume :
187
Issue :
7
Database :
OpenAIRE
Journal :
Mikrochimica acta
Accession number :
edsair.doi.dedup.....4169a8dd57817008474441e41cc3ee1f