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Screen-printed electrodes modified with carbon black and polyelectrolyte films for determination of cancer marker carbohydrate antigen 19-9
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2020
-
Abstract
- Electrochemical immunosensors have been developed to determine the carbohydrate antigen 19-9 (CA19-9). They are based on screen-printed carbon electrodes (SPCEs) coated with layer-by-layer (LbL) films of carbon black (CB) and polyelectrolytes. Owing to a suitable choice of LbL film architecture, the procedures for immobilization of anti-CA19-9 antibodies on the electrode surfaces were straightforward. Mechanically flexible immunosensors were capable of detecting CA19-9 within a dynamic range of 0.01 to 40 U mL−1 and a limit of detection of 0.07 U mL−1 using differential pulse voltammetry. In addition to detecting CA19-9 at clinically relevant concentrations for pancreatic cancer in standard solutions, the immunosensors provide the determination of CA19-9 on cell lysate and human serum samples. Using LbL films led to immunosensors with superior performance compared to similar systems obtained by drop casting. The fabrication of this relatively simple, inexpensive platform is a demonstration that SPCEs modified with cost-effective materials are able to detect cancer biomarkers and may be adapted to other disposable immunosensors.
- Subjects :
- Materials science
FILMES FINOS
Nanochemistry
Nanotechnology
02 engineering and technology
Standard solution
Electrochemistry
01 natural sciences
Analytical Chemistry
Soot
Limit of Detection
Biomarkers, Tumor
Humans
Antigens, Tumor-Associated, Carbohydrate
Electrodes
Detection limit
Immunoassay
010401 analytical chemistry
Carbon black
Electrochemical Techniques
021001 nanoscience & nanotechnology
Polyelectrolytes
Polyelectrolyte
0104 chemical sciences
Electrode
Differential pulse voltammetry
0210 nano-technology
Antibodies, Immobilized
Subjects
Details
- ISSN :
- 14365073
- Volume :
- 187
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Mikrochimica acta
- Accession number :
- edsair.doi.dedup.....4169a8dd57817008474441e41cc3ee1f