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Quantification of EVI1 transcript levels in acute myeloid leukemia by RT-qPCR analysis: A study by the ALFA Group
- Source :
- Leukemia Research. 39:1443-1447
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- EVI1 overexpression confers poor prognosis in acute myeloid leukemia (AML). Quantification of EVI1 expression has been mainly assessed by real-time quantitative PCR (RT-qPCR) based on relative quantification of EVI1-1D splice variant. In this study, we developed a RT-qPCR assay to perform quantification of EVI1 expression covering the different splice variants. A sequence localized in EVI1 exons 14 and 15 was cloned into plasmids that were used to establish RT-qPCR standard curves. Threshold values to define EVI1 overexpression were determined using 17 bone marrow (BM) and 31 peripheral blood (PB) control samples and were set at 1% in BM and 0.5% in PB. Samples from 64 AML patients overexpressing EVI1 included in the ALFA-0701 or -0702 trials were collected at diagnosis and during follow-up (n=152). Median EVI1 expression at AML diagnosis was 23.3% in BM and 3.6% in PB. EVI1 expression levels significantly decreased between diagnostic and post-induction samples, with an average variation from 21.6% to 3.56% in BM and from 4.0% to 0.22% in PB, but did not exceed 1 log10 reduction. Our study demonstrates that the magnitude of reduction in EVI1 expression levels between AML diagnosis and follow-up is not sufficient to allow sensitive detection of minimal residual disease.
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Neoplasm, Residual
Adolescent
Transcription, Genetic
Bone Marrow Cells
Biology
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Young Adult
Exon
Plasmid
Proto-Oncogenes
medicine
Humans
Multicenter Studies as Topic
splice
RNA, Messenger
RNA, Neoplasm
Aged
Randomized Controlled Trials as Topic
Blood Cells
Gene Expression Regulation, Leukemic
Alternative splicing
Myeloid leukemia
Hematology
Middle Aged
Minimal residual disease
Molecular biology
MDS1 and EVI1 Complex Locus Protein
Neoplasm Proteins
DNA-Binding Proteins
Leukemia, Myeloid, Acute
Real-time polymerase chain reaction
medicine.anatomical_structure
Oncology
Female
Bone marrow
Follow-Up Studies
Transcription Factors
Subjects
Details
- ISSN :
- 01452126
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Leukemia Research
- Accession number :
- edsair.doi.dedup.....415d32611ece5c8a73ea6da5dd88274b