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Histone Deacetylase 2 in Sudden Sensorineural Hearing Loss Patients in Response to Intratympanic Methylprednisolone Perfusion

Authors :
Yanhong Dai
Xiaoping Du
Qiongqiong Zhou
Jie Hou
Wandong She
Lisheng Xie
Source :
Otolaryngology–Head and Neck Surgery. 154:164-170
Publication Year :
2015
Publisher :
Wiley, 2015.

Abstract

Objective To evaluate the expression of histone deacetylase 2 (HDAC2) in peripheral blood mononuclear cells (PBMCs) from patients with sudden sensorineural hearing loss (SSNHL) who were refractory to systemic glucocorticoid treatment and to identify the relationship between the level of HDAC2 and glucocorticoid insensitivity. Study design Prospective clinical study. Setting This study was conducted in Nanjing Drum Tower Hospital, Nanjing University Medical School. Subjects and methods PBMCs were collected from 42 refractory SSNHL patients. After a 10-day intratympanic methylprednisolone perfusion (IMP) and systemic Ginkgo biloba extract treatment, the SSNHL patients were divided into 2 groups according to their hearing recovery after IMP (IMP sensitive and insensitive). Real-time polymerase chain reaction and HDAC2 protein assays were used to detect the relative expression levels of HDAC2 in PBMCs. The HDAC2 mRNA expression and protein levels in PBMCs collected from 17 volunteers were used as normal HDAC2 reference levels. Results Compared with normal reference levels, HDAC2 protein levels were significantly reduced, while the HDAC2 mRNA expression was much higher in all refractory SSNHL patients before IMP. HDAC2 mRNA expression and HDAC2 protein levels were significantly elevated in the IMP-sensitive group, while no change was observed in the IMP-insensitive group after IMP plus systemic antioxidant treatment. Conclusions Reduced HDAC2 protein levels may be 1 of the mechanistic underpinnings of corticosteroid insensitivity in refractory SSNHL patients. IMP can increase HDAC2 protein levels and the expression of HDAC2 mRNA in IMP-sensitive patients. HDAC2 protein levels might be regulated through posttranslational modifications.

Details

ISSN :
10976817 and 01945998
Volume :
154
Database :
OpenAIRE
Journal :
Otolaryngology–Head and Neck Surgery
Accession number :
edsair.doi.dedup.....415c1ff422ee984fadaea32f2985e60e