Back to Search Start Over

The Interaction of Curcumin with Phospholipid Model Membranes. a Study using Differential Scanning Calorimetry, NMR, X-Ray Diffraction and Infrared Spectroscopy

Authors :
Juan C. Gómez-Fernández
Alessio Ausili
Ángel Pérez-Lara
Ana M. deGodos
Francisco J. Aranda
Senena Corbalán-García
Source :
Biophysical Journal. 98(3)
Publication Year :
2010
Publisher :
Elsevier BV, 2010.

Abstract

Curcumin is a polyphenol present in turmeric, widely used in Asian traditional medicine and cooking, which has many and diverse biological effects and is found incorporated in membranes. We have studied the mode in which curcumin modulates the physical properties of 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) multilamellar membranes and 1, 2-dielaidoyl-sn-glycero-3-phosphoetnanolamine (DEPE). Curcumin disordered DPPC membranes at temperatures below Tc as seen through DSC, FT-IR, 2H-NMR, WAXD and SAXD. The decrease induced in Tc, suggests that curcumin is oriented in the bilayer with its main axis parallel to the acyl chains. Above Tc curcumin also introduced disordering as seen by FT-IR. FT-IR also showed that curcumin alters the conformation of the polar group of DPPC, increasing the percentage of unhydrated C=O groups, but however it does not form hydrogen bonds with neither the C=O group nor the phosphate group of DPPC. SAXD showed a remarkable increase in the repeating spacings by the presence of curcumin probably indicating the formation of a ripple phase. A partial phase diagram was built, which suggest the formation of a phospholipid/curcumin complex given place to immiscibilities in both the fluid and the rigid states, between curcumin and DPPC. Additionally DEPE was used to test the effect of curcumin on its polymorphism, and it was found that the temperature at which HII phase is formed was decreased, indicating that curcumin favours negative curvature of the membrane, which may be important to explain its effect on membrane dynamics and on membrane proteins or on proteins which may be activated through membrane insertion.

Details

ISSN :
00063495
Volume :
98
Issue :
3
Database :
OpenAIRE
Journal :
Biophysical Journal
Accession number :
edsair.doi.dedup.....41585c8d355fe4fc50329a8b11e7225f
Full Text :
https://doi.org/10.1016/j.bpj.2009.12.2604