Back to Search Start Over

Inhibition of lipid peroxidation by paraquat: Site of inhibition in the cytochrome P-450-dependent steroid hydroxylase system from bovine adrenal cortex mitochondria

Authors :
Jerzy Klimek
Tokuji Kimura
A. Paul Schaap
Source :
Biochemical and Biophysical Research Communications. 107:499-505
Publication Year :
1982
Publisher :
Elsevier BV, 1982.

Abstract

We have found that NADPH-dependent lipid peroxidation in bovine adrenal cortex mitochondria is strongly inhibited by paraquat. The site of the inhibition of the lipid peroxidation by paraquat has been examined. Paraquat neither inhibits NADPH-2,6-dichlorophenolindophenol nor NADPH-cytochrome c reductase activities. However, paraquat is able to retard the rate of reduction of cytochrome P-450 by NADPH. The spectrophotometric measurements provide the first evidence that lipid peroxidation in adrenal cortex mitochondria involves cytochrome P-450 and that the inhibitory effect of paraquat on lipid peroxidation is due to reoxidation of reduced cytochrome P-450 by the reagent.

Details

ISSN :
0006291X
Volume :
107
Database :
OpenAIRE
Journal :
Biochemical and Biophysical Research Communications
Accession number :
edsair.doi.dedup.....41553991b9767da594370878137a16b7
Full Text :
https://doi.org/10.1016/0006-291x(82)91519-4