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Inhibition of lipid peroxidation by paraquat: Site of inhibition in the cytochrome P-450-dependent steroid hydroxylase system from bovine adrenal cortex mitochondria
- Source :
- Biochemical and Biophysical Research Communications. 107:499-505
- Publication Year :
- 1982
- Publisher :
- Elsevier BV, 1982.
-
Abstract
- We have found that NADPH-dependent lipid peroxidation in bovine adrenal cortex mitochondria is strongly inhibited by paraquat. The site of the inhibition of the lipid peroxidation by paraquat has been examined. Paraquat neither inhibits NADPH-2,6-dichlorophenolindophenol nor NADPH-cytochrome c reductase activities. However, paraquat is able to retard the rate of reduction of cytochrome P-450 by NADPH. The spectrophotometric measurements provide the first evidence that lipid peroxidation in adrenal cortex mitochondria involves cytochrome P-450 and that the inhibitory effect of paraquat on lipid peroxidation is due to reoxidation of reduced cytochrome P-450 by the reagent.
- Subjects :
- Paraquat
inorganic chemicals
Lipid Peroxides
medicine.medical_specialty
Cytochrome
Biophysics
Reductase
Biochemistry
Lipid peroxidation
chemistry.chemical_compound
Cytochrome P-450 Enzyme System
Internal medicine
medicine
Animals
heterocyclic compounds
Molecular Biology
NADPH-Ferrihemoprotein Reductase
Dose-Response Relationship, Drug
Metyrapone
biology
Adrenal cortex
Cell Biology
Malondialdehyde
Aminoglutethimide
Butanones
Mitochondria
Endocrinology
medicine.anatomical_structure
chemistry
Steroid hydroxylase
biology.protein
Cattle
Oxidation-Reduction
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....41553991b9767da594370878137a16b7
- Full Text :
- https://doi.org/10.1016/0006-291x(82)91519-4