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Two-carrier influx of neutral amino acids into rabbit ileal mucosa
- Publication Year :
- 1979
-
Abstract
- 1. The influx of serine, alanine and methionine across the brush border membrane of the rabbit ileal mucosa has been measured during short periods of incubation. 2. A kinetic analysis of the uptake data, assuming one mediated entry mechanism or one mediated entry mechanism plus a diffusion component to be present, does not provide an adequate explanation for the results obtained. Methionine inhibition of serine uptake provided direct evidence that the diffusive entry of serine into the rabbit ileum was small or non-existent. 3. Data taken from amino acid inhibition and substrate-uptake experiments, fitted simultaneously to a double hyperbolic model of amino acid uptake, give good agreement between predicted and experimental results. There is also good quantitative agreement between computer-derived kinetic constants in the present work and similar constants obtained previously using a different method of analysis. 4. Present work supports the general hypothesis that neutral amino acids use two mediated pathways to enter the rabbit ileal mucosa. The possible physiological significance of these results and their probable effect on currently held concepts of how amino acids cross the brush border membrane of the rabbit intestinal mucosa is discussed.
- Subjects :
- Brush border
Physiology
Direct evidence
Ileum
Biology
Models, Biological
Serine
chemistry.chemical_compound
Methionine
Intestinal mucosa
medicine
Animals
Intestinal Mucosa
Alanine
chemistry.chemical_classification
Microvilli
Biological Transport
Amino acid
Kinetics
medicine.anatomical_structure
chemistry
Biochemistry
Rabbits
Extracellular Space
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....414d793cd13bce4f09c5c98baca9c44d