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Effects of glial cell line-derived neurotrophic factor on dental pulp cells
- Publication Year :
- 2011
- Publisher :
- Sage, 2011.
-
Abstract
- This study investigated the effects of glial cell line-derived neurotrophic factor (GDNF) on dental pulp cells (DPCs). Cultures of DPCs expressed GDNF as well as its receptors, GFRα1 and RET. Addition of recombinant GDNF to cultures in serum-containing medium did not significantly affect DPC growth; however, GDNF dose-dependently increased viable cell number under serum-free culture conditions. Live/dead, lactate dehydrogenase (LDH), and caspases-3/-7 assays demonstrated that cell death occurred under serum-free conditions, and that GDNF significantly reduced the number of dead cells by inhibiting apoptotic cell death. GDNF also stimulated cell proliferation in serum-free conditions, as assessed by the BrdU incorporation assay. The effect of GDNF was abolished in the presence of inhibitors to GFRα1 and RET suggesting receptor-mediated events. This study also demonstrated that GDNF counteracted TNFα-induced DPC cytotoxicity, suggesting that GDNF may be cytoprotective under disease conditions. In conclusion, our findings indicate that GDNF promotes cell survival and proliferation of DPCs and suggest that GDNF may play a multifunctional role in the regulation of dental pulp homeostasis.
- Subjects :
- Male
Programmed cell death
Glial Cell Line-Derived Neurotrophic Factor Receptors
Cell Survival
animal diseases
Cell
Apoptosis
Biology
Culture Media, Serum-Free
Neurotrophic factors
medicine
Glial cell line-derived neurotrophic factor
Animals
Humans
Glial Cell Line-Derived Neurotrophic Factor
Rats, Wistar
Receptor
General Dentistry
Cells, Cultured
Dental Pulp
Cell Proliferation
Cell growth
Tumor Necrosis Factor-alpha
urogenital system
RK Dentistry
Proto-Oncogene Proteins c-ret
Cell biology
Rats
medicine.anatomical_structure
nervous system
Immunology
biology.protein
GDNF family of ligands
Subjects
Details
- Language :
- English
- ISSN :
- 15440591
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....414a73af581801231fe5aab5cf0055e2