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Class A scavenger receptor-1/2 facilitates the uptake of bovine milk exosomes in murine bone marrow-derived macrophages and C57BL/6J mice
- Source :
- Am J Physiol Cell Physiol
- Publication Year :
- 2021
- Publisher :
- American Physiological Society, 2021.
-
Abstract
- Bovine milk exosomes (BMEs) are being explored in drug delivery despite their rapid elimination by macrophages. We aimed at identifying the BME transporter in murine bone marrow-derived macrophages (BMDMs). Fluorophore-labeled BMEs were used in transport studies in BMDMs from C57BL/6J and class A scavenger receptor type 1/2 (CASR-1/2) knockout mice and tissue accumulation in macrophage-depleted C57BL/6J mice. Parametric and nonparametric statistics tests for pairwise and multiple comparisons were used. Chemical inhibitors of phagocytosis by cytochalasin D led to a 69 ± 18% decrease in BME uptake compared with controls ( P < 0.05), whereas inhibitors of endocytic pathways other than phagocytosis had a modest effect on uptake ( P > 0.05). Inhibitors of class A scavenger receptors (CASRs) including CASR-1/2 caused a 70% decrease in BME uptake ( P < 0.05). The uptake of BMEs by BMDMs from CASR-1/2 knockout mice was smaller by 58 ± 23% compared with wild-type controls ( P < 0.05). Macrophage depletion by clodronate caused a more than 44% decrease in BME uptake in the spleen and lungs ( P < 0.05), whereas the decrease observed in liver was not statistically significant. In conclusion, CASR-1/2 facilitates the uptake of BMEs in BMDMs and C57BL/6J mice.
- Subjects :
- Male
Bovine milk
Cytochalasin D
Physiology
Phagocytosis
Gene Expression
Exosomes
C57bl 6j
Mice
medicine
Animals
Protein Isoforms
Scavenger receptor
Lung
Fluorescent Dyes
Mice, Knockout
Staining and Labeling
Chemistry
Macrophages
Scavenger Receptors, Class A
Transporter
Cell Biology
Endocytosis
Microvesicles
Cell biology
Mice, Inbred C57BL
Milk
medicine.anatomical_structure
Liver
Drug delivery
Cattle
Female
Bone marrow
Clodronic Acid
Spleen
Research Article
Subjects
Details
- ISSN :
- 15221563 and 03636143
- Volume :
- 321
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Cell Physiology
- Accession number :
- edsair.doi.dedup.....4135755ef26ca0b6b5c3344c94596625
- Full Text :
- https://doi.org/10.1152/ajpcell.00222.2021