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Biodegradation efficiency of functionally important populations selected for bioaugmentation in phenol- and oil-polluted area
- Source :
- FEMS Microbiology Ecology. 51:363-373
- Publication Year :
- 2005
- Publisher :
- Oxford University Press (OUP), 2005.
-
Abstract
- Denaturing gradient gel electrophoresis of amplified fragments of genes coding for 16S rRNA and for the largest subunit of multicomponent phenol hydroxylase (LmPH) was used to monitor the behaviour and relative abundance of mixed phenol-degrading bacterial populations (Pseudomonas mendocina PC1, P. fluorescens strains PC18, PC20 and PC24) during degradation of phenolic compounds in phenolic leachate- and oil-amended microcosms. The analysis indicated that specific bacterial populations were selected in each microcosm. The naphthalene-degrading strain PC20 was the dominant degrader in oil-amended microcosms and strain PC1 in phenolic leachate microcosms. Strain PC20 was not detectable after cultivation in phenolic leachate microcosms. Mixed bacterial populations in oil-amended microcosms aggregated and formed clumps, whereas the same bacteria had a planktonic mode of growth in phenolic leachate microcosms. Colony hybridisation data with catabolic gene specific probes indicated that, in leachate microcosms, the relative proportions of bacteria having meta (PC1) and ortho (PC24) pathways for degradation of phenol and p-cresol changed alternately. The shifts in the composition of mixed population indicated that different pathways of metabolism of aromatic compounds dominated and that this process is an optimised response to the contaminants present in microcosms.
- Subjects :
- DNA, Bacterial
Electrophoresis
Bioaugmentation
Molecular Sequence Data
Population
Pseudomonas fluorescens
DNA, Ribosomal
Polymerase Chain Reaction
Applied Microbiology and Biotechnology
Microbiology
Mixed Function Oxygenases
Phenols
Pseudomonas
RNA, Ribosomal, 16S
education
Ecosystem
education.field_of_study
Ecology
biology
Biodegradation
Plankton
biology.organism_classification
Culture Media
Biodegradation, Environmental
Petroleum
Environmental chemistry
Microcosm
Temperature gradient gel electrophoresis
Bacteria
Pseudomonas mendocina
Subjects
Details
- ISSN :
- 15746941 and 01686496
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Ecology
- Accession number :
- edsair.doi.dedup.....412d0c5032fd1a2e0c729f9df902fe2f