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Time course study of the response to LPS targeting the pig immune gene networks
- Source :
- BMC Genomics, BMC Genomics, BioMed Central, 2017, 18, ⟨10.1186/s12864-017-4363-5⟩, BMC Genomics, Vol 18, Iss 1, Pp 1-14 (2017), BMC Genomics (18), . (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Background Stress is a generic term used to describe non-specific responses of the body to all kinds of challenges. A very large variability in the response can be observed across individuals, depending on numerous conditioning factors like genetics, early influences and life history. As a result, there is a wide range of individual vulnerability and resilience to stress, also called robustness. The importance of robustness-related traits in breeding strategies is increasing progressively towards the production of animals with a high level of production under a wide range of climatic conditions and management systems, together with a lower environmental impact and a high level of animal welfare. The present study aims at describing blood transcriptomic, hormonal, and metabolic responses of pigs to a systemic challenge using lipopolysaccharide (LPS). The objective is to analyze the individual variation of the biological responses in relation to the activity of the HPA axis measured by the levels of plasma cortisol after LPS and ACTH in 120 juvenile Large White (LW) pigs. The kinetics of the response was measured with biological variables and whole blood gene expression at 4 time points. A multilevel statistical analysis was used to take into account the longitudinal aspect of the data. Results Cortisol level reaches its peak 4 h after LPS injection. The characteristic changes of white blood cell count to LPS were observed, with a decrease of total count, maximal at t=+4 h, and the mirror changes in the respective proportions of lymphocytes and granulocytes. The lymphocytes / granulocytes ratio was maximal at t=+1 h. An integrative statistical approach was used and provided a set of candidate genes for kinetic studies and ongoing complementary studies focused on the LPS-stimulated inflammatory response. Conclusions The present study demonstrates the specific biomarkers indicative of an inflammation in swine. Furthermore, these stress responses persist for prolonged periods of time and at significant expression levels, making them good candidate markers for evaluating the efficacy of anti-inflammatory drugs. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-4363-5) contains supplementary material, which is available to authorized users.
- Subjects :
- Lipopolysaccharides
Male
0301 basic medicine
Candidate gene
Stress
Hypothalamic
pituitary-adrenal (HPA) axis
Cortisol
Time-course
Systems biology
Microarray
Pig
PORCINE PBMCS
SIGNIFICANTLY INFLUENCES
TRANSCRIPTOMIC ANALYSIS
MOLECULAR-GENETICS
GROWING PIGS
EXPRESSION
INNATE
SWINE
LIPOPOLYSACCHARIDE
ACTIVATION
Hydrocortisone
Lipopolysaccharide
Swine
[SDV]Life Sciences [q-bio]
chemistry.chemical_compound
0302 clinical medicine
Gene Regulatory Networks
[MATH]Mathematics [math]
Hypothalamic-pituitary-adrenal (HPA) axis
Whole blood
2. Zero hunger
medicine.anatomical_structure
Female
medicine.symptom
Research Article
Biotechnology
medicine.drug
lcsh:QH426-470
lcsh:Biotechnology
Inflammation
Biology
03 medical and health sciences
lcsh:TP248.13-248.65
White blood cell
Genetics
medicine
Animals
[INFO]Computer Science [cs]
[SDV.GEN]Life Sciences [q-bio]/Genetics
Gene Expression Profiling
Immunity
Blood Cell Count
Gene expression profiling
lcsh:Genetics
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
Kinetics
030104 developmental biology
chemistry
Immunology
Transcriptome
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14712164
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....412b64ea2f559a059ad841cf1fb22e36