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Filarial glutathione-S-transferase: a potential vaccine candidate against lymphatic filariasis
- Source :
- Vaccine. 26(32)
- Publication Year :
- 2008
-
Abstract
- Present report enumerates the vaccine potential of a glutathione-S-transferase purified from Setaria cervi against lymphatic filariasis. In jirds (Meriones unguiculatus) vaccination trial, a very significant 82.75% (p < 0.005) reduction in adult parasite burden was observed in ScGST immunized group after 90 days post Brugia malayi L3 challenge. An inverse correlation between the antibody level and worm burden was found in ScGST immunized group (Person’s correlation r = 0.943, p < 0.05). No recoveries of worms were obtained in heart and lungs of vaccinated group. The Antibodies reactive to ScGST appeared within four weeks of first dose and were able to neutralize the GST activity up to 86%. In an earlier study we have shown vaccine potential of ScGST against B. malayi by ADCC. Evaluation of cytokine profile in T-cells isolated from BALB/c mice immunized with ScGST were also showed predominance of Th2 response which further maintained the humoral immunity generated by ScGST administration in mice. The overall observations prompted us to envisage ScGST as a potential vaccine candidate against lymphatic filariasis.
- Subjects :
- Male
Protozoan Vaccines
T-Lymphocytes
Helminthiasis
Antibodies, Protozoan
Brugia malayi
Mice
Elephantiasis, Filarial
medicine
Animals
Lymphatic filariasis
Cell Proliferation
Glutathione Transferase
Antibody-dependent cell-mediated cytotoxicity
Mice, Inbred BALB C
General Veterinary
General Immunology and Microbiology
biology
Public Health, Environmental and Occupational Health
biology.organism_classification
medicine.disease
Virology
Vaccination
Infectious Diseases
Immunology
Humoral immunity
Setaria cervi
biology.protein
Molecular Medicine
Cytokines
Female
Antibody
Gerbillinae
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 26
- Issue :
- 32
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....412b43468adf43cc3a80562fb94fc017