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Protective Effects of Paeoniflorin Against Glutamate-Induced Neurotoxicity in PC12 Cells via Antioxidant Mechanisms and Ca2+ Antagonism
- Source :
- Cellular and Molecular Neurobiology. 30:1059-1066
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Preclinical and clinical investigations have shown hippocampal neuronal atrophy and destruction were observed in patients with depression, which could be ameliorated by the treatment with antidepressants. Therefore, neuroprotection has been proposed to be one of the acting mechanisms of antidepressant. Paeoniflorin, a monoterpene glycoside, has been reported to display antidepressant-like effects in animal models of behavioral despair. The present study aimed to examine the protective effect of paeoniflorin on glutamate-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. The results showed that pretreatment with paeoniflorin elevated cell viability, inhibited apoptosis, decreased levels of intracellular reactive oxygen species and malondialdehyde, and enhanced activity of superoxide dismutase in glutamate-treated PC12 cells. Pretreatment with paeoniflorin also reversed the increased intracellular Ca(2+) concentration and the reduced Calbindin-D28K mRNA level caused by glutamate in PC12 cells. The results suggest that paeoniflorin exerts a neuroprotective effect on glutamate-induced neurotoxicity in PC12 cells, at least in part, via inhibiting oxidative stress and Ca(2+) overload. This neuroprotective effect may be one of the action pathways accounting for the in vivo antidepressant activity of paeoniflorin.
- Subjects :
- Bridged-Ring Compounds
Calbindins
Cell Survival
Glutamic Acid
Pharmacology
medicine.disease_cause
Benzoates
PC12 Cells
Neuroprotection
Antioxidants
Superoxide dismutase
Cellular and Molecular Neuroscience
chemistry.chemical_compound
S100 Calcium Binding Protein G
Glucosides
Malondialdehyde
medicine
Animals
Humans
Viability assay
Molecular Structure
biology
Superoxide Dismutase
Anti-Inflammatory Agents, Non-Steroidal
Glutamate receptor
Neurotoxicity
Cell Biology
General Medicine
Paeoniflorin
medicine.disease
Antidepressive Agents
Rats
Oxidative Stress
Neuroprotective Agents
chemistry
Calbindin 1
Monoterpenes
biology.protein
Calcium
Neurotoxicity Syndromes
Reactive Oxygen Species
Oxidative stress
Subjects
Details
- ISSN :
- 15736830 and 02724340
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Cellular and Molecular Neurobiology
- Accession number :
- edsair.doi.dedup.....411750531598d7d8655721477ba3d416