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Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua
- Source :
- Cell Reports, Vol 35, Iss 7, Pp 109138-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Summary: Various human diseases and pregnancy-related disorders reflect endometrial dysfunction. However, rodent models do not share fundamental biological processes with the human endometrium, such as spontaneous decidualization, and no existing human cell cultures recapitulate the cyclic interactions between endometrial stromal and epithelial compartments necessary for decidualization and implantation. Here we report a protocol differentiating human pluripotent stem cells into endometrial stromal fibroblasts (PSC-ESFs) that are highly pure and able to decidualize. Coculture of PSC-ESFs with placenta-derived endometrial epithelial cells generated organoids used to examine stromal-epithelial interactions. Cocultures exhibited specific endometrial markers in the appropriate compartments, organization with cell polarity, and hormone responsiveness of both cell types. Furthermore, cocultures recapitulate a central feature of the human decidua by cyclically responding to hormone withdrawal followed by hormone retreatment. This advance enables mechanistic studies of the cyclic responses that characterize the human endometrium.
- Subjects :
- Pluripotent Stem Cells
0301 basic medicine
Hormone Responsive
Cell type
Stromal cell
QH301-705.5
Biology
General Biochemistry, Genetics and Molecular Biology
Endometrium
03 medical and health sciences
epithelial-stromal signaling
0302 clinical medicine
Cell polarity
medicine
Humans
human pluripotent stem cells
Biology (General)
Induced pluripotent stem cell
cyclic hormone response
Decidua
Decidualization
Fibroblasts
Coculture Techniques
Cell biology
030104 developmental biology
medicine.anatomical_structure
Female
endometrial stromal cells
Stromal Cells
Cell-cell signaling
cell-cell signaling
030217 neurology & neurosurgery
decidua
Subjects
Details
- Language :
- English
- ISSN :
- 22111247
- Volume :
- 35
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....4115ba21eaa13574d5acc587caa41c6b