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Diversity and evolution of cytochrome P450s of Jacobaea vulgaris and Jacobaea aquatica
- Source :
- BMC Plant Biology, 20, 342, BMC Plant Biology, BMC Plant Biology, Vol 20, Iss 1, Pp 1-14 (2020)
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Background Collectively, plants produce a huge variety of secondary metabolites (SMs) which are involved in the adaptation of plants to biotic and abiotic stresses. The most characteristic feature of SMs is their striking inter- and intraspecific chemical diversity. Cytochrome P450 monooxygenases (CYPs) often play an important role in the biosynthesis of SMs and thus in the evolution of chemical diversity. Here we studied the diversity and evolution of CYPs of two Jacobaea species which contain a characteristic group of SMs namely the pyrrolizidine alkaloids (PAs). Results We retrieved CYPs from RNA-seq data of J. vulgaris and J. aquatica, resulting in 221 and 157 full-length CYP genes, respectively. The analyses of conserved motifs confirmed that Jacobaea CYP proteins share conserved motifs including the heme-binding signature, the PERF motif, the K-helix and the I-helix. KEGG annotation revealed that the CYPs assigned as being SM metabolic pathway genes were all from the CYP71 clan but no CYPs were assigned as being involved in alkaloid pathways. Phylogenetic analyses of full-length CYPs were conducted for the six largest CYP families of Jacobaea (CYP71, CYP76, CYP706, CYP82, CYP93 and CYP72) and were compared with CYPs of two other members of the Asteraceae, Helianthus annuus and Lactuca sativa, and with Arabidopsis thaliana. The phylogenetic trees showed strong lineage specific diversification of CYPs, implying that the evolution of CYPs has been very fast even within the Asteraceae family. Only in the closely related species J. vulgaris and J. aquatica, CYPs were found often in pairs, confirming a close relationship in the evolutionary history. Conclusions This study discovered 378 full-length CYPs in Jacobaea species, which can be used for future exploration of their functions, including possible involvement in PA biosynthesis and PA diversity.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Jacobaea vulgaris
Plant Science
urologic and male genital diseases
01 natural sciences
Evolution, Molecular
03 medical and health sciences
Cytochrome P-450 Enzyme System
Phylogenetics
lcsh:Botany
heterocyclic compounds
Senecio
KEGG
Phylogeny
Pyrrolizidine Alkaloids
Plant Proteins
Genetics
biology
Phylogenetic tree
Pyrrolizidine alkaloid biosynthesis
organic chemicals
Jacobaea
Cytochrome P450
Biodiversity
respiratory system
biology.organism_classification
lcsh:QK1-989
Conserved motifs
enzymes and coenzymes (carbohydrates)
Metabolic pathway
030104 developmental biology
biology.protein
Chemical diversity
Jacobaea aquatica
RNA-seq
Research Article
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14712229
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology
- Accession number :
- edsair.doi.dedup.....410fec60916554a834a694191c0dbcfb