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Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation
- Source :
- International Journal of Molecular Sciences, Vol 19, Iss 8, p 2322 (2018), International Journal of Molecular Sciences, Volume 19, Issue 8
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Background: Three-dimensional (3D) culture changes cell characteristics and function, suggesting that 3D culture provides a more physiologically relevant environment for cells compared with 2D culture. We investigated the differences in cell functions depending on the culture model in human trophoblast cells (Sw.71). Methods: Sw.71 cells were incubated in 2D monolayers or simple 3D spheroids. After incubation, cells were corrected to assess RNA-seq transcriptome or protein expression, and culture medium were corrected to detect cytokines. To clarify the role of actin cytoskeleton, spheroid Sw.71 cells were treated mycalolide B (inhibitor of actin polymerization) in a 3D culture. Results: RNA-seq transcriptome analysis, results revealed that 3D-cultured cells had a different transcriptional profile compared with 2D-cultured cells, especially regarding inflammation-related molecules. Although interleukin-6 (IL-6) mRNA level was higher in 3D-culured cells, its secretion levels were higher in 2D-cultured cells. In addition, the levels of mRNA and protein expression of regnase-1, regulatory RNase of inflammatory cytokine, significantly increased in 3D culture, suggesting post-translational modification of IL-6 mRNA via regnase-1. Treatment with mycalolide B reduced cell-to-cell contact to build 3D formation and increased expression of actin cytoskeleton, resulting in increased IL-6 secretin. Conclusion: Cell dimensionality plays an essential role in governing the spatiotemporal cellular outcomes, including inflammatory cytokine production and its negative regulation associated with regnase-1.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Cell
Cell Culture Techniques
Inflammation
Microtubules
Article
Catalysis
Inorganic Chemistry
Transcriptome
lcsh:Chemistry
03 medical and health sciences
Ribonucleases
0302 clinical medicine
Spheroids, Cellular
medicine
Humans
RNA, Messenger
Physical and Theoretical Chemistry
Cytoskeleton
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Actin
Base Sequence
Chemistry
interleukin-6
Organic Chemistry
Trophoblast
General Medicine
Actin cytoskeleton
trophoblast cells
Trophoblasts
Computer Science Applications
Cell biology
Actin Cytoskeleton
030104 developmental biology
medicine.anatomical_structure
Cytokine
Gene Expression Regulation
regnase-1
lcsh:Biology (General)
lcsh:QD1-999
inflammation
030220 oncology & carcinogenesis
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 19
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....410f687f0fd3b22352dec0ef620f76a2