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Histone H3 Interacts and Colocalizes with the Nuclear Shuttle Protein and the Movement Protein of a Geminivirus
- Source :
- Journal of Virology. 85:11821-11832
- Publication Year :
- 2011
- Publisher :
- American Society for Microbiology, 2011.
-
Abstract
- Geminiviruses are plant-infecting viruses with small circular single-stranded DNA genomes. These viruses utilize nuclear shuttle proteins (NSPs) and movement proteins (MPs) for trafficking of infectious DNA through the nuclear pore complex and plasmodesmata, respectively. Here, a biochemical approach was used to identify host factors interacting with the NSP and MP of the geminivirus Bean dwarf mosaic virus (BDMV). Based on these studies, we identified and characterized a host nucleoprotein, histone H3, which interacts with both the NSP and MP. The specific nature of the interaction of histone H3 with these viral proteins was established by gel overlay and in vitro and in vivo coimmunoprecipitation (co-IP) assays. The NSP and MP interaction domains were mapped to the N-terminal region of histone H3. These experiments also revealed a direct interaction between the BDMV NSP and MP, as well as interactions between histone H3 and the capsid proteins of various geminiviruses. Transient-expression assays revealed the colocalization of histone H3 and NSP in the nucleus and nucleolus and of histone H3 and MP in the cell periphery and plasmodesmata. Finally, using in vivo co-IP assays with a Myc-tagged histone H3, a complex composed of histone H3, NSP, MP, and viral DNA was recovered. Taken together, these findings implicate the host factor histone H3 in the process by which an infectious geminiviral DNA complex forms within the nucleus for export to the cell periphery and cell-to-cell movement through plasmodesmata.
- Subjects :
- Cytoplasm
DNA, Plant
Molecular Sequence Data
Immunology
Plasmodesma
Biology
Microbiology
Histones
Histone H3
chemistry.chemical_compound
Solanum lycopersicum
Histone H1
Virology
Protein Interaction Mapping
Tobacco
Histone H2A
Immunoprecipitation
Protein Interaction Domains and Motifs
Nuclear pore
Movement protein
Cell Nucleus
Plasmodesmata
Nuclear Proteins
Sequence Analysis, DNA
Molecular biology
Virus-Cell Interactions
Nucleoprotein
Plant Viral Movement Proteins
chemistry
Begomovirus
Insect Science
Host-Pathogen Interactions
Capsid Proteins
Cell Nucleolus
DNA
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....4106d7c63eb10f1bd202321cb3d4f243
- Full Text :
- https://doi.org/10.1128/jvi.00082-11