Supplementary Figure S5 from miR-629 Targets TRIM33 to Promote TGFβ/Smad Signaling and Metastatic Phenotypes in ccRCC

Supplementary Figure S5. A, Caki-2 cells were transfected with the miR-629 inhibitor or control miRNA inhibitor for 72 h, and then protein samples were collected and subjected to western blot analysis with anti-HNF4α antibody. The membrane was re-probed with the anti-β-tubulin antibody. The relative HNF4α expression is shown as mean {plus minus} S.D. of three independent experiments. B, Protein samples from ccRCC cell lines were collected and subjected to western blot analysis for HNF4α. The relative HNF4α expression is shown as means {plus minus} S.D. of three independent experiments. (C, D) The expression of HNF4α in ccRCC specimens was examined by immunohistochemical staining. One normal kidney tissue and 17 ccRCC samples were examined.