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Clinical evaluation of the NS1 antigen-capture ELISA for early diagnosis of dengue virus infection in Brazil
- Source :
- Journal of Medical Virology. 82:1400-1405
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- The fact that the diagnosis of infection with dengue virus is usually made by detecting IgM antibodies during the convalescent phase of the disease interferes with disease management and, consequently, with reducing mortality rates. This study evaluated the sensitivity and specificity of detection of NS1 in samples of patients suspected of acute dengue virus infection in Brazil. The results were used to institute treatment and the sensitivity and specificity of detection of NS1 were compared to the results of detection of IgM, virus isolation, and RT-PCR. Detection of NS1 yielded better results than RT-PCR and virus isolation. When considering IgM detection and RT-PCR positive results as "gold standards," the sensitivity and specificity of the NS1 assay were 95.9% and 81.1%, respectively. All patients enrolled in the study were treated promptly and had an uneventful course of the disease. The detection of NS1 provided better results than the diagnostic techniques used currently during the acute phase of disease (RT-PCR and virus isolation). Detection of NS1 is an important tool for the diagnosis of acute dengue infection, particularly in highly endemic areas, allowing for rapid treatment of patients and reduction of disease burden.
- Subjects :
- Adult
Male
viruses
Enzyme-Linked Immunosorbent Assay
Disease
Dengue virus
Biology
Antibodies, Viral
medicine.disease_cause
Sensitivity and Specificity
Dengue fever
Dengue
Virology
medicine
Humans
Disease burden
Viral Structural Proteins
Reverse Transcriptase Polymerase Chain Reaction
Mortality rate
Dengue Virus
Middle Aged
medicine.disease
Early Diagnosis
Infectious Diseases
Real-time polymerase chain reaction
Immunoglobulin M
Immunology
biology.protein
RNA, Viral
Female
Viral disease
Brazil
Subjects
Details
- ISSN :
- 01466615
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Virology
- Accession number :
- edsair.doi.dedup.....40fee3daa4e5cd6e02efe54a74a0e250