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Novel polysaccharide from Chaenomeles speciosa seeds: Structural characterization, α-amylase and α-glucosidase inhibitory activity evaluation
- Source :
- International journal of biological macromolecules. 153
- Publication Year :
- 2020
-
Abstract
- Purification and structural characterization of a novel polysaccharide fraction from Chaenomeles speciosa seeds were investigated. After hot water extraction and ethanol precipitation, the crude polysaccharide was sequentially purified with Cellulose DEAE-52 and gel-filtration chromatography, and a highly purified polysaccharide fraction (F3) was obtained. The structure of F3 was characterized by high-performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), ultraviolet-visible (UV), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectrum, together with methylation, scanning electron microscopy (SEM), atomic force microscope (AFM), and Congo-red test analysis. The results indicated that F3 was a homogeneous polysaccharide fraction with a molecular weight of 8.65 × 106 Da, and it was composed of Rha, GlcA, Gal, and Ara in a molar ratio of 6.34:5.73:47.14:40.13. The backbone of F3 was consisted of →3,6)-Galp-(1→, and the side chains of F3 were composed of Araf-(1→, →4)-GlcpA-(1→, →4)-Galp-(1→ and →3)-Rhap-(1→. The hypoglycemic assays demonstrated F3 had good α-amylase and α-glucosidase inhibition activities, and their IC50 values were 6.24 mg/mL and 4.59 mg/mL respectively. Thus, the polysaccharide from Chaenomeles speciose could be applied as a potential natural source in retarding postprandial hyperglycemia effects.
- Subjects :
- 02 engineering and technology
Polysaccharide
Biochemistry
High-performance liquid chromatography
Gel permeation chromatography
03 medical and health sciences
chemistry.chemical_compound
Structural Biology
Polysaccharides
Glycoside Hydrolase Inhibitors
Amylase
Chaenomeles speciosa
Cellulose
Molecular Biology
Rosaceae
Ethanol precipitation
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Chromatography
biology
Chemistry
alpha-Glucosidases
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
Fruit
biology.protein
alpha-Amylases
0210 nano-technology
Chaenomeles
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....40f923986f07ac23d0cac8d9e5cd6ffe