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Aspirin inhibits proliferation and promotes differentiation of neuroblastoma cells via p21Waf1 protein up-regulation and Rb1 pathway modulation
- Source :
- Journal of Cellular and Molecular Medicine (Online) (2019): 7073–7087. doi:10.1111/jcmm.14610, info:cnr-pdr/source/autori:Pozzoli G 1,2, Petrucci G 1, Navarra P 1,2, Marei HE 3, Cenciarelli C 4/titolo:Aspirin inhibits proliferation and promotes differentiation of neuroblastoma cells via p21Waf1 protein up-regulation and Rb1 pathway modulation./doi:10.1111%2Fjcmm.14610/rivista:Journal of Cellular and Molecular Medicine (Online)/anno:2019/pagina_da:7073/pagina_a:7087/intervallo_pagine:7073–7087/volume
- Publication Year :
- 2019
- Publisher :
- University Press "Carol Davila", Bucharest , Romania, 2019.
-
Abstract
- Several clinical and experimental studies have demonstrated that regular use of aspirin (acetylsalicylic acid, ASA) correlates with a reduced risk of cancer and that the drug exerts direct anti-tumour effects. We have previously reported that ASA inhibits proliferation of human glioblastoma multiforme-derived cancer stem cells. In the present study, we analysed the effects of ASA on nervous system-derived cancer cells, using the SK-N-SH (N) human neuroblastoma cell line as an experimental model. ASA treatment of SK-N-SH (N) dramatically reduced cell proliferation and motility, and induced neuronal-like differentiation, indicated by the appearance of the neuronal differentiation marker tyrosine hydroxylase (TH) after 5 days. ASA did not affect cell viability, but caused a time-dependent accumulation of cells in the G0 /G1 phase of the cell cycle, with a concomitant decrease in the percentage of cells in the G2 phase. These effects appear to be mediated by a COX-independent mechanism involving an increase in p21Waf1 and underphosphorylated retinoblastoma (hypo-pRb1) protein levels. These findings may support a potential role of ASA as adjunctive therapeutic agent in the clinical management of neuroblastoma.
- Subjects :
- 0301 basic medicine
Settore BIO/14 - FARMACOLOGIA
aspirin
03 medical and health sciences
neuroblastoma
0302 clinical medicine
Cancer stem cell
Neuroblastoma
medicine
Viability assay
Rb1
neuronal differentiation
Tyrosine hydroxylase
Chemistry
Cell growth
cell cycle
Cancer
Cell Biology
Cell cycle
medicine.disease
030104 developmental biology
030220 oncology & carcinogenesis
Cancer cell
acetylsalicylic acid
Cancer research
Molecular Medicine
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine (Online) (2019): 7073–7087. doi:10.1111/jcmm.14610, info:cnr-pdr/source/autori:Pozzoli G 1,2, Petrucci G 1, Navarra P 1,2, Marei HE 3, Cenciarelli C 4/titolo:Aspirin inhibits proliferation and promotes differentiation of neuroblastoma cells via p21Waf1 protein up-regulation and Rb1 pathway modulation./doi:10.1111%2Fjcmm.14610/rivista:Journal of Cellular and Molecular Medicine (Online)/anno:2019/pagina_da:7073/pagina_a:7087/intervallo_pagine:7073–7087/volume
- Accession number :
- edsair.doi.dedup.....40e4e8a44bef7780647af9e2ef3dd465