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Wnt/β-Catenin and Estrogen Signaling Converge in Vivo
- Source :
- Journal of Biological Chemistry. 279:40255-40258
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Wnt and estrogen signaling represent important regulatory pathways, each controlling a wide range of biological processes. While an increasing number of observations suggest potential convergence between these pathways, no direct evidence of their functional interaction has been reported. Using human colon and breast cancer cells, we found that estrogen receptor (ER) alpha- and beta-catenin precipitated within the same immunocomplexes, reciprocally enhanced the transactivation of cognate reporter genes, and were reciprocally recruited to cognate response elements in the promoters of endogenous target genes. Using transgenic Drosophila that ectopically expressed human ERalpha alone or together with metabolically stable beta-catenin/Armadillo mutants, we demonstrated genetic interaction between these signal transducers in vivo. Thus, we present here the first direct evidence of cross-talk between Wnt and estrogen signaling pathways via functional interaction between beta-catenin and ERalpha.
- Subjects :
- Transcriptional Activation
Transcription, Genetic
Transgene
Immunoblotting
Estrogen receptor
Apoptosis
Biology
Transfection
Biochemistry
Transactivation
Genes, Reporter
Cell Line, Tumor
In Situ Nick-End Labeling
Animals
Humans
Transgenes
Promoter Regions, Genetic
Molecular Biology
beta Catenin
Estrogen receptor beta
Genetics
Reporter gene
Estrogen Receptor alpha
Wnt signaling pathway
Estrogens
Promoter
Cell Biology
Precipitin Tests
Chromatin
Cell biology
Cytoskeletal Proteins
Receptors, Estrogen
Catenin
Mutation
Trans-Activators
Drosophila
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 279
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....40dd4755a81891d5d502483d34940675