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Bilateral vagotomy or atropine pre-treatment reduces experimental diesel-soot induced lung inflammation
- Source :
- Toxicology and applied pharmacology. 219(1)
- Publication Year :
- 2006
-
Abstract
- To investigate the role of the vagus nerve in acute inflammatory and cardiorespiratory responses to diesel particulate (DP) in the rat airway, we measured changes in respiration, blood pressure and neutrophils in lungs of urethane anesthetized Wistar rats 6-h post-instillation of DP (500 {mu}g) and studied the effect of mid-cervical vagotomy or atropine (1 mg kg{sup -1}) pre-treatment. In conscious rats, we investigated DP, with and without atropine pre-treatment. DP increased neutrophil level in BAL (bronchoalveolar lavage) fluid from intact anesthetized rats to 2.5 {+-} 0.7 x 10{sup 6} cells (n = 8), compared with saline instillation (0.3 {+-} 0.1 x 10{sup 6}, n = 7; P < 0.05). Vagotomy reduced DP neutrophilia to 0.8 {+-} 0.2 x 10{sup 6} cells (n = 8; P < 0.05 vs. intact); atropine reduced DP-induced neutrophilia to 0.3 {+-} 0.2 x 10{sup 6} (n = 4; P < 0.05). In conscious rats, DP neutrophilia of 8.5 {+-} 1.8 x 10{sup 6}, n = 4, was reduced by pre-treatment with atropine to 2.2 {+-} 1.2 x 10{sup 6} cells, n = 3. Hyperventilation occurred 6 h after DP in anesthetized rats with intact vagi, but not in bilaterally vagotomized or atropine pre-treated animals andmore » was abolished by vagotomy (P < 0.05, paired test). There were no significant differences in the other variables (mean blood pressure, heart rate and heart rate variability) measured before and 360 min after DP. In conclusion, DP activates a pro-inflammatory vago-vagal reflex which is reduced by atropine. Muscarinic ACh receptors in the rat lung are involved in DP-induced neutrophilia, and hence muscarinic antagonists may reduce airway and/or cardiovascular inflammation evoked by inhaled atmospheric DP in susceptible individuals.« less
- Subjects :
- Atropine
Male
medicine.medical_specialty
Pulmonary Circulation
Neutrophils
medicine.medical_treatment
Blood Pressure
Muscarinic Antagonists
Vagotomy
Toxicology
Heart Rate
Internal medicine
Muscarinic acetylcholine receptor
medicine
Animals
Respiratory system
Rats, Wistar
Vehicle Emissions
Pharmacology
Vagovagal reflex
business.industry
Macrophages
Pneumonia
Neutrophilia
Vagus nerve
Rats
Endocrinology
Data Interpretation, Statistical
Acute Disease
Respiratory Mechanics
medicine.symptom
business
Bronchoalveolar Lavage Fluid
Acetylcholine
medicine.drug
Subjects
Details
- ISSN :
- 0041008X
- Volume :
- 219
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Toxicology and applied pharmacology
- Accession number :
- edsair.doi.dedup.....40d90d34f3a92c218187ba0775ffb0f4