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Cloning, expression and functional characterization of rat napsin
- Source :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1492:207-210
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- A full-length cDNA clone coding for rat napsin was identified by homology search of the ZooSeq rat EST database (Incyte). Northern blot analysis revealed high expression of napsin mRNA transcripts in kidney, lung and spleen. Western blot analysis showed that rat napsin is expressed in kidney as a 50-kDa, highly glycosylated, monomeric protein. Lysates prepared from human embryonic kidney cells (HEK293) transfected with rat napsin showed increased enzymatic activity which was inhibited by pepstatin.
- Subjects :
- DNA, Complementary
Databases, Factual
Blotting, Western
Molecular Sequence Data
Biophysics
Biology
Kidney
Biochemistry
Homology (biology)
chemistry.chemical_compound
Western blot
Structural Biology
Endopeptidases
Genetics
medicine
Animals
Aspartic Acid Endopeptidases
Humans
Amino Acid Sequence
Northern blot
Cloning, Molecular
Cells, Cultured
Expressed Sequence Tags
Messenger RNA
Base Sequence
Sequence Homology, Amino Acid
medicine.diagnostic_test
HEK 293 cells
Transfection
Blotting, Northern
Molecular biology
Rats
medicine.anatomical_structure
Gene Expression Regulation
chemistry
Pepstatin
Subjects
Details
- ISSN :
- 01674781
- Volume :
- 1492
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
- Accession number :
- edsair.doi.dedup.....40c36c9ac281a29fa57099abfab53500
- Full Text :
- https://doi.org/10.1016/s0167-4781(00)00069-5