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Unexpected dose response of HaCaT to UVB irradiation
- Source :
- In Vitro Cellular & Developmental Biology - Animal. 54:589-599
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Application of high-dosage UVB irradiation in phototherapeutic dermatological treatments present health concerns attributed to UV-exposure. In assessing UV-induced photobiological damage, we investigated dose-dependent effects of UVB irradiation on human keratinocyte cells (HaCaT). Our study implemented survival and apoptosis assays and revealed an unexpected dose response wherein higher UVB-dosage induced higher viability. Established inhibitors, such as AKT- (LY294002), PKC- (Gö6976, and Rottlerin), ERK- (PD98059), P38 MAPK- (SB203580), and JNK- (SP600125), were assessed to investigate UV-induced apoptotic pathways. Despite unobvious contributions of known signaling pathways in dose-response mediation, microarray analysis identified transcriptional expression of UVB-response genes related to the respiratory-chain. Observed correlation of ROS-production with UVB irradiation potentiated ROS as the underlying mechanism for observed dose responses. Inability of established pathways to explain such responses suggests the complex nature underlying UVB-phototherapy response.
- Subjects :
- Keratinocytes
0301 basic medicine
MAPK/ERK pathway
Transcription, Genetic
Cell Survival
Ultraviolet Rays
p38 mitogen-activated protein kinases
Apoptosis
Nitric Oxide
Cell Line
Electron Transport
Phosphatidylinositol 3-Kinases
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Superoxides
Humans
Benzopyrans
LY294002
Protein kinase B
Flavonoids
integumentary system
Gene Expression Profiling
Cell Cycle
Acetophenones
Dose-Response Relationship, Radiation
Cell Biology
General Medicine
Up-Regulation
HaCaT
030104 developmental biology
chemistry
Cancer research
Poly(ADP-ribose) Polymerases
Signal transduction
Reactive Oxygen Species
Proto-Oncogene Proteins c-akt
Rottlerin
Developmental Biology
Subjects
Details
- ISSN :
- 1543706X and 10712690
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- In Vitro Cellular & Developmental Biology - Animal
- Accession number :
- edsair.doi.dedup.....40a49cbd77745124ec6e082970d7a653